| 高活性木质素降解菌株T-8的分离、筛选与鉴定 |
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| 引用本文： | 李成翠,李术娜,朱宝成.高活性木质素降解菌株T-8的分离、筛选与鉴定[J].河北农业大学学报,2010,33(6). |
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| 作者姓名： | 李成翠 李术娜 朱宝成 |
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| 作者单位： | 河北农业大学,生命科学学院,河北,保定,071001 |
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| 基金项目： | 河北省农业科技成果转化项目(09825125D) |
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| 摘 要： | 为了筛选对木质素有降解能力的菌株,本试验从菜地土壤、动物粪便、青贮饲料中分离筛选出79株产芽孢细菌,初筛获得具有木质素降解能力的菌株20株,经过复筛,筛选出一株具有较高木质素降解能力的菌株T-8,其木质素过氧化物酶酶活为626.67 U/L。对T-8进行形态鉴定、生理生化特征鉴定和16S rDNA序列分析,鉴定该菌株为解淀粉芽孢杆菌(Bacillus amyloliquefaciens),其与B. amyloliquefaciens的相似性高达99.91%。
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| 关 键 词： | 木质素 降解 筛选 鉴定 |
Isolation,screening and identification of the strain T-8 with high lignin-degrading activity |
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| LI Cheng-cui,LI Shu-na,ZHU Bao-cheng.Isolation,screening and identification of the strain T-8 with high lignin-degrading activity[J].Journal of Agricultural University of Hebei,2010,33(6). |
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| Authors: | LI Cheng-cui LI Shu-na ZHU Bao-cheng |
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| Institution: | LI Cheng-cui,LI Shu-na,ZHU Bao-cheng(College of Life Science,Agricultural University of Hebei,Baoding 071001,China) |
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| Abstract: | In order to obtain the strain with lignin-degrading capability,79 spore-forming bacterial strains were isolated and screened from specimen collected from the vegetable field,animal manure and silage feed.Total 20 strains with lignin-degrading capability were obtained through the primary screening.Among them,a strain named T-8 was found with higher activity of degrading lignin than other strains via the secondary screening,and the activity of lignin peroxidase was 626.67 U/L.The morphology characteristics,ph... |
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| Keywords: | lignin degradation screening identification |
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