| 部分枣属植物硅胶干燥叶片DNA提取方法的比较 |
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| 引用本文： | 李学营,彭建营,彭士琪.部分枣属植物硅胶干燥叶片DNA提取方法的比较[J].河北农业大学学报,2006,29(1):38-40. |
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| 作者姓名： | 李学营 彭建营 彭士琪 |
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| 作者单位： | 河北农业大学,园艺学院,河北,保定,071001 |
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| 基金项目： | 国家自然科学基金资助项目(30270927)，河北省自然科学基金项目(C2004000363) |
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| 摘 要： | 以硅胶干燥15 D和半年的3种野生枣属植物叶片为试材,分别采用简易CTAB法、高盐沉淀法和高盐低PH法3种方法提取基因组总DNA,通过A260/A280、琼脂糖凝胶电泳、PCR扩增分析对提取的基因组DNA进行了鉴定。半年内不同保存时期的材料对于DNA提取没有明显差异;高盐沉淀法所得DNA纯度最高。
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| 关 键 词： | 枣属植物 叶片 硅胶保存 基因组DNA 提取方法 |
| 文章编号： | 1000-1573（2006）01-0038-03 |
| 收稿时间： | 2005-04-13 |
| 修稿时间： | 2005-04-13 |
Comparison between extracting methods for genomic DNA of dry leaf of Ziziphus Mill. saved in silica |
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| LI Xue-ying,PENG Jian-ying,PENG Shi-qi.Comparison between extracting methods for genomic DNA of dry leaf of Ziziphus Mill. saved in silica[J].Journal of Agricultural University of Hebei,2006,29(1):38-40. |
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| Authors: | LI Xue-ying PENG Jian-ying PENG Shi-qi |
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| Institution: | College of Horticulture, Agricultural University of Hebei, Baoding 071001, China |
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| Abstract: | Improved CTAB,high salt precipitation,and high salt and low pH method were employed to extract genomic DNA from the leaves of 3 species of Ziziphus Mill.saved in silica for 15 days and for half a year.The extracted DNA was tested using the value of A_(260)/A_(280),agarose gel electrophoresis and PCR amplification.The DNA from the leaves saved in silica for half a year is good for PCR amplification and the method of high salt precipitation is best. |
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| Keywords: | Ziziphus Mill leaves preservation in silica genomic DNA extracting method |
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