| 人工合成小麦对白粉病的抗性评价 |
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| 引用本文： | 张宁,陆鸣,宋凤景,赵紫慧,黄江,吴龙飞,王晓鸣,李洪杰.人工合成小麦对白粉病的抗性评价[J].河北农业大学学报,2013(1):6-10. |
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| 作者姓名： | 张宁 陆鸣 宋凤景 赵紫慧 黄江 吴龙飞 王晓鸣 李洪杰 |
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| 作者单位： | 河北科技师范学院生命科技学院;中国农业科学院作物科学研究所农作物基因资源与基因改良国家重大科学工程;中国科学院遗传与发育生物学研究所农业资源研究中心 |
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| 基金项目： | 中央级公益性科研院所基本科研业务费专项(20105);国家小麦产业技术体系专项(CARS-3-1);国家转基因生物新品种培育科技部重大专项(2009ZX08002-006B)的资助 |
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| 摘 要： | 本研究利用我国不同地区的20个小麦白粉菌菌株对28份合成小麦材料进行抗病性评价,并利用与基因连锁的分子标记检测这些合成小麦中Pm2抗白粉病基因。在供试的合成小麦材料中,C7、C14和C2分别抗14～16个菌株,另有6份材料能抗10个以上菌株,C16和C20不抗任何菌株。来自相同杂交组合的材料抗病性表现有很大不同。根据分子检测的结果,C9和C19的扩增片段与Pm2基因相同,但是抗谱分析表明这两个合成小麦对白粉菌的反应型与Pm2基因不同。
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| 关 键 词： | 合成小麦 白粉病 抗病基因 |
Assessment of reaction to powdery mildew in synthetic wheat |
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| ZHANG Ning,LU Ming,SONG Feng-jing,ZHAO Zi-hui,HUANG Jiang,WU Long-fei,WANG Xiao-ming,LI Hong-jie.Assessment of reaction to powdery mildew in synthetic wheat[J].Journal of Agricultural University of Hebei,2013(1):6-10. |
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| Authors: | ZHANG Ning LU Ming SONG Feng-jing ZHAO Zi-hui HUANG Jiang WU Long-fei WANG Xiao-ming LI Hong-jie |
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| Institution: | 1.Hebei Normal University of Science and Technology,Qinhuangdao 066004,China;2.Institute of Crop Sciences, Chinese Academy of Agricultural Sciences,National Key Facility for Crop Gene Resources and Genetic Improvement, Beijing 100081,China;3.Center for Agricultural Resources Research, Institute of Genetics and Developmental Biology,Chinese Academy of Sciences,Shijiazhuang 050021,China.) |
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| Abstract: | This study was conducted to assess reactions of 28 synthetic wheat accessions to 20 isolates of Blumeria gramins(DC.) f.sp.tritici E.O.Speer,the causal agent of powdery mildew in wheat(Triticum aestivum L.).The gene-specific marker Xcfd81 was used to detect gene Pm2 for resistance to powdery mildew.Among the synthetic wheat accessions tested,accessions C7,C14,and C2 were resistant to 14-16 isolates,and another 6 accessions were resistant to more than 10 isolates.Accessions C16 and C20 were susceptible to all of isolates tested.Different reactions to powdery mildew were observed in the synthetic accessions derived from the same crosses.Accessions C9 and C19 produced the same amplification results as Pm2,but the reactions of these synthetic accessions differed from that of Pm2. |
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| Keywords: | synthetic wheat powdery mildew resistance gene |
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