| EMS处理对草莓离体叶片再生植株耐盐性的影响 |
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| 引用本文： | 刘艳萌,张学英,葛会波,胡淑明.EMS处理对草莓离体叶片再生植株耐盐性的影响[J].河北农业大学学报,2006,29(6):25-28,48. |
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| 作者姓名： | 刘艳萌 张学英 葛会波 胡淑明 |
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| 作者单位： | 河北农业大学,园艺学院,河北,保定,071001 |
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| 基金项目： | 河北农业大学9816、博士启动基金资助(200501) |
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| 摘 要： | 为了给草莓抗逆诱变育种提供技术和方法,以草莓继代苗为试材,用甲基磺酸乙酯(EMS)对其叶片进行处理后,叶片的存活率和再生率比对照均有所下降,再生植株以0.8%NaCl为选择压力进行初步筛选后,超氧化物歧化酶(SOD)、过氧化物酶(POD)活性增强,丙二醛(MDA)含量减少,游离脯氨酸含量增加,对盐渍环境表现出一定的抗性。
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| 关 键 词： | EMS 草莓 诱变 耐盐鉴定 |
| 文章编号： | 1000-1573（2006）06-0025-04 |
| 收稿时间： | 2006-04-04 |
| 修稿时间： | 2006-04-04 |
The effects of EMS on salt tolerance of regenerated plants from strawberry leaf in vitro |
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| LIU Yan-meng,ZHANG Xue-ying,GE Hui-bo,HU Shu-ming.The effects of EMS on salt tolerance of regenerated plants from strawberry leaf in vitro[J].Journal of Agricultural University of Hebei,2006,29(6):25-28,48. |
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| Authors: | LIU Yan-meng ZHANG Xue-ying GE Hui-bo HU Shu-ming |
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| Institution: | College of Horticulture, Agricultural University of Hebei, Baoding 071001, China |
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| Abstract: | The leaf of strawberry in vitro culture was treated by EMS,both survival rate and regeneration rate decreased more than that of control.Sodium chloride tolerant variants were selected from the regenerated plants which were cultured on 0.8% saline medium,then the induced mutant can resist salt stress by increasing SODase,PODase activities,content of free proline and decreasing content of MDA. |
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| Keywords: | EMS strawberry induced mutation salt-tolerance identification |
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