| M13分子标记快速区分树木溃疡病原真菌的种类 |
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| 引用本文： | 王庆灵,崔建州,赵嘉平.M13分子标记快速区分树木溃疡病原真菌的种类[J].河北农业大学学报,2013(1):74-79. |
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| 作者姓名： | 王庆灵 崔建州 赵嘉平 |
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| 作者单位： | 河北农业大学林学院;中国林业科学研究院林业新技术研究所遗传育种国家重点开放试验室 |
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| 基金项目： | 科技基础性工作专项“中国树木溃疡病原多样性及其生态地理分布和危害调查”(2009FY210100) |
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| 摘 要： | 树木溃疡病菌具有寄主多样性、危害症状复杂且分布广泛的特点,快速简便地区分真菌类别是杨树溃疡病研究及防治中需要解决的重要问题。在对树木溃疡病菌的遗传多样性研究,发现以M13为引物的PCR扩增可以在不同的种类之间产生稳定分化的带型。因此,本研究分离了43株18种树木溃疡病相关真菌,并且对这些菌株进行rDNA-ITS序列测定及M13-PCR。结果显示,M13带型差异与真菌的特定种类相对应。因此,认为M13分子标记可以准确快速地鉴定、区分树木溃疡病菌。
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| 关 键 词： | 树木溃疡病 引物M13 分子标记 rDNA内转录间隔区 |
M13 molecular marker technique rapidly distinguish pathogenic fungal group causing tree canker |
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| WANG Qing-ling,CUI Jian-zhou,ZHAO Jia-ping.M13 molecular marker technique rapidly distinguish pathogenic fungal group causing tree canker[J].Journal of Agricultural University of Hebei,2013(1):74-79. |
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| Authors: | WANG Qing-ling CUI Jian-zhou ZHAO Jia-ping |
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| Institution: | 1.College of Forestry,Agricultural University of Hebei,Baoding 071001,China; 2.Research Institute of Forestry New Technology,Genetic Breeding National Laboratory,Beijing 100091,China) |
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| Abstract: | Pathogenic fungal group causing tree canker has a variety of hosts,complex harm syptom and a wide distribution.Simply and conveniently identifying fungal group is an important problem in the study of prevention and control of tree canker.During studying genetic diversity of tree caker pathogens,primer M13 PCR produced stable and differential bands in differential species.Therefore,to verify the differential ability of M13,this study separated 43 isolates of tree caker related fungi,which were determined by rDNA-ITS sequences and M13-PCR of the isolates.The result proved that M13 band diversity was corresponds with specific species.The results suggest that M13 molecular marker technique can distinguish different canker pathogens accurately and rapidly. |
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| Keywords: | tree canker primer M13 molecular marker technique rDNA-ITS |
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