一株拮抗细菌HJ5高产抗菌物质发酵条件优化

从黄顶菊茎中分离得到的内生细菌H J5,是一株具有拮抗玉米大斑病菌活性的枯草芽孢杆菌.研究发现,H J5菌株通过产生并分泌某种胞外物质发挥拮抗活性.为了增强HJ5菌株发酵产拮抗物质的能力,对HJ5菌株发酵条件进行单因子试验以及正交试验,从而确定了H J5菌株发酵产抗菌活性物质的最佳培养基配方为1％葡萄糖作为碳源、1％大豆蛋白胨作为氮源、0.01％ MnC12作为添加无机盐,初始pH为8.对HJ5菌株发酵工艺参数优化结果为:50/250mL的装液量、2％的接种量、28℃180 r/min振荡培养66h.经发酵条件优化后较基础培养基,HJ5菌株拮抗活性增强了近1倍.

The optimal fermentation conditions of HJ5 for high production of antimicrobial substance

Setosphaeria turcica was inhibited by HJ5 which was an endophytic bacterium isolated from the stem of Flaveria bidentis.The optimum medium were 1% of glucose,1% of soy peptone,0.01% of MnCl2 and primary pH was 8.0 via single factor tests and orthogonal test.The rotating speed of rocking incubator was 180 r/min.The inoculum and liquid volume in flask were 2% and 50 mL/250 mL,respectively.The cultivating temperature was 28℃through optimizing the fermentation technological parameter.After the optimization,antagonistic activity of HJ5 enhances nearly doubled.