| 拟南芥AtCCaP2基因缺失突变体的鉴定及表型观察 |
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| 引用本文： | 高亢,马惠斌,吴立柱,潘延云.拟南芥AtCCaP2基因缺失突变体的鉴定及表型观察[J].河北农业大学学报,2012(5):67-71. |
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| 作者姓名： | 高亢 马惠斌 吴立柱 潘延云 |
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| 作者单位： | 河北农业大学生命科学学院 |
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| 基金项目： | 国家自然科学基金项目(No.30570993);河北省自然基金项目(C2008000292) |
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| 摘 要： | 本研究拟利用反向遗传学研究AtCCaP2基因的功能。通过PCR及RT-PCR的方法筛选和鉴定了拟南芥AtCCaP2基因的T-DNA插入突变纯合体atccap2,该纯合体AtCCaP2基因表达缺失。RT-PCR分析显示该基因在拟南芥雌雄蕊等生殖器官中有较多的表达。通过对生长表型的观察发现atccap2突变体出现早花现象,抽薹时间较野生型早3d,显示该基因可能参与了拟南芥的早花表型。
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| 关 键 词： | 拟南芥 AtCCaP 反向遗传学 |
Identification and phenotype of T-DNA insert AtCCaP2 gene mutant in Arabidopsis thaliana |
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| GAO Kang,MA Hui-bin,WU Li-zhu,PAN Yan-yun.Identification and phenotype of T-DNA insert AtCCaP2 gene mutant in Arabidopsis thaliana[J].Journal of Agricultural University of Hebei,2012(5):67-71. |
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| Authors: | GAO Kang MA Hui-bin WU Li-zhu PAN Yan-yun |
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| Institution: | (College of Life Science,Agricultural University of Hebei,Baoding 071001,China) |
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| Abstract: | In this study,the function of AtCCaP2 gene was characterized using reverse genetics method.The T-DNA insert AtCCaP2 gene mutants were identified by PCR and RT-PCR,and the homozygote mutant atccap2 was isolated.RT-PCR analyses showed that AtCCaP2 gene were higher expression in stamen and pistil than in leaf.The atccap2 mutants were early bolting 3 days than the wild type.These result showed that AtCCaP2 gene might cause early flowering phenotype in Arabidopsis. |
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| Keywords: | Arabidopsis thaliana AtCCaP reversed genetics |
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