拟南芥干旱敏感突变体的筛选及其对干旱胁迫的响应

采用质量浓度为5 g/L的PEG - 6000进行渗透胁迫,利用弯根法筛选拟南芥T-DNA插入突变体库,得到干旱敏感突变体36 -1.该突变体在PEG - 6000渗透胁迫下,种子萌发率、根系长度均低于野生型；盆栽试验表明:水分正常条件下,突变体36 -1与野生型幼苗形态、根冠比、叶片含水量等并无明显差异；干旱条件下,...

Screening of drought-sensitive mutant in Arabidopsis thaliana and responses of drought-sensitive mutant to drought stress

The drought-sensitive mutant 36-1 plant was obtained under growing T-DNA inserted Arabidopsis thaliana on 1/2 MS medium containing 5% PEG-6000 by observing root tip bending.The seed germination rate,root length of the mutant 36-1were lower than wild-type under planting or growing on 1/2 MS medium containing PEG-6000.The seedling shape,root/ shoot ratio and relative water content of the leaves between mutant 36-1 and wild-type were not obviously different under growing in pot with normal soil water content.The root/ shoot ratio,relative water content of the leaves in mutant 36-1 were obviously lower than in wild-type and the leaves in mutant 36-1 seedling wilt earlier compared with wild-type seedlings under growing in drought conditions.The water loss of the leaves detached from either mutant 36-1 or wild-type seedlings was not obviously different during the first 4 h course of the test and after that the water loss of the leaves detached from mutant 36-1 was beginning obviously higher than wild-type leaves.The flanking sequences of T-DNA inserted site was amplified by TAIL-PCR.Sequence alignment proved that T-DNA inserted the seventh exon of the AT2g06025.RT-PCR confirmed that the expression of the AT2g06025 in mutant 36-1 was obviously lower than wild-type Arabidopsis thaliana.