| 日本沼虾卵黄磷蛋白生化性质分析 |
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| 引用本文： | 穆淑梅,康现江,李彦芹,郭明申,温秀荣.日本沼虾卵黄磷蛋白生化性质分析[J].河北农业大学学报,2006,29(5):72-75. |
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| 作者姓名： | 穆淑梅 康现江 李彦芹 郭明申 温秀荣 |
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| 作者单位： | 河北大学,生命科学学院,河北,保定,071002 |
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| 基金项目： | 河北大学青年基金(2005Q13)，河北省教委项目(97120)资助 |
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| 摘 要： | 采用电泳洗脱法从成熟日本沼虾卵巢中提纯卵黄磷蛋白,并初步分析了其生化性质。聚丙烯酰胺凝胶电泳(PAGE)和SDS-聚丙烯酰胺凝胶电泳(SDS-PAGE)结果显示:其相对分子量为458 ku;由3个多肽亚基组成,分子量分别为110 ku,96 ku,89 ku,且三亚基上均有糖辅基的结合;样品缓冲液中β-巯基乙醇的有无并不影响亚基的分离,推测亚基间不存在二硫键。
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| 关 键 词： | 日本沼虾 卵黄磷蛋白 生化性质 |
| 文章编号： | 1000-1573（2006）05-0072-04 |
| 收稿时间： | 2006-05-12 |
| 修稿时间： | 2006-05-12 |
Analysis on the characterization of vitellin from the mature ovary of Macrobrachium nipponense |
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| MU Shu-mei,KANG Xian-jiang,LI Yan-qin,GUO Ming-shen,WEN Xiu-rong.Analysis on the characterization of vitellin from the mature ovary of Macrobrachium nipponense[J].Journal of Agricultural University of Hebei,2006,29(5):72-75. |
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| Authors: | MU Shu-mei KANG Xian-jiang LI Yan-qin GUO Ming-shen WEN Xiu-rong |
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| Institution: | The College of Life Science, Hebei University, Baoding 071002, China |
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| Abstract: | With the PAGE and various staining methods, one form of vitellin(Vn) from the mature ovary of Macrobrachium nipponense was examined.And then,Vn was purified by electric-elution.Using a gradient native PAGE,Vn was found to have a molecular mass of 458 ku.Three subunits(110, 96,and 89 ku) in purified Vn were detected with SDS-PAGE.The three subunits were all glycolpolypep-tides,and nondisulfide bonds were found in the binding of the polypeptide subunits. |
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| Keywords: | Macrobrachium nipponense vitellin characterization |
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