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| 两种纯化苏云金杆菌HD-73毒素蛋白方法的比较研究 | |
| 引用本文： | 宋萍,潘映红,陆秀君,王勤英,张凤巧.两种纯化苏云金杆菌HD-73毒素蛋白方法的比较研究[J].河北农业大学学报,2005,28(3):63-65. |
| 作者姓名： | 宋萍 潘映红 陆秀君 王勤英 张凤巧 |
| 作者单位： | 1. 河北农业大学,植物保护学院,河北,保定,071001 2. 中国农科院,植物保护研究所,北京,100094 3. 河北农业大学,图书馆,河北,保定,071001 |
| 基金项目： | 国家自然科学基金重点项目(30130130)，河北农业大学青年基金项目 |
| 摘 要： | 以苏云金杆菌HD 73伴胞晶体为活性蛋白,经胰蛋白酶酶解后分别用阴离子交换柱分离纯化法和等电点纯化法进行纯化,纯化后得到的样品进行SDS-PAGE分析,结果表明,两种分离纯化都获得67kDa的毒素蛋白,但是离子交换柱纯化法样品消耗量大,得率低;而等电点沉淀法样品消耗少,得率高。 |
| 关 键 词： | BtCry1Ac 离子交换柱 等电点沉淀 SDS-PAGE |
| 文章编号： | 1000-1573(2005)03-0063-03 |
Comparative study of two purification methods of Bacillus thuringiensis HD-73 toxin protein |
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| SONG Ping,PAN Ying-hong,LU Xiu-jun,WANG Qin-ying,Zhang Feng-qiao.Comparative study of two purification methods of Bacillus thuringiensis HD-73 toxin protein[J].Journal of Agricultural University of Hebei,2005,28(3):63-65. | |
| Authors: | SONG Ping PAN Ying-hong LU Xiu-jun WANG Qin-ying Zhang Feng-qiao |
| Institution: | SONG Ping~1,PAN Ying-hong~2,LU Xiu-jun~1,WANG Qin-ying~1,ZHANG Feng-qiao~3 |
| Abstract: | Studies were carried out about purification of HD-73 toxin protein by two methods, toxin was converted by trypsin-treated from HD-73 pro-toxin, then purified by an anion-exchange column and pH dependent precipitation method. Samples of purified toxin were analyzed with SDS-PAGE. The results are as follows: 67 kDa toxin protein was obtained by two methods, but anion-exchange column has high wastage and low productivity, on the contray to other method. |
| Keywords: | Bt Cry1Ac anion-exchange column pH dependent precipitation SDS-PAGE |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |