| 猪笼草高效再生体系的建立 |
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| 引用本文： | 郑文永,张洁,杨权,赵少荣,王阳,王冬梅.猪笼草高效再生体系的建立[J].河北农业大学学报,2010,33(6). |
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| 作者姓名： | 郑文永 张洁 杨权 赵少荣 王阳 王冬梅 |
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| 作者单位： | 河北农业大学,生命科学学院,河北,保定,071001 |
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| 基金项目： | 河北农业大学大学生科技创新活动基金(2010SM03) |
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| 摘 要： | 以猪笼草(Nepenthes mirabilis)腋芽为试材,研究不同浓度无机盐离子、蔗糖以及BA和NAA对猪笼草不定芽增殖和生根的影响。结果表明:最佳增殖培养基为1/2 MS基础培养基附加30 g/L蔗糖、2.0 mg/L BA和0.2 mg/L NAA;生根培养基以1/4 MS基础培养基附加0.3 g/L活性炭、15 g/L蔗糖、1.5 mg/L IBA和0.5mg/L NAA为最佳,猪笼草生根效果最好,生根率达到92%。
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| 关 键 词： | 猪笼草 离体快繁 增殖 生根 |
Establishment of effective regeneration system of Nepenthes mirabilis |
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| ZHENG Wen-yong,ZHANG Jie,YANG Quan,ZHAO Shao-rong,WANG Yang,WANG Dong-mei.Establishment of effective regeneration system of Nepenthes mirabilis[J].Journal of Agricultural University of Hebei,2010,33(6). |
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| Authors: | ZHENG Wen-yong ZHANG Jie YANG Quan ZHAO Shao-rong WANG Yang WANG Dong-mei |
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| Institution: | ZHENG Wen-yong,ZHANG Jie,YANG Quan,ZHAO Shao-rong,WANG Yang,WANG Dong-mei(College of Life Science,Agricultural University of Hebei,Baoding 071001,China) |
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| Abstract: | The axillary buds of Nepenthes mirabilis were used as specimens.The effect of different concentration inorganic salt,sucrose,BA,IBA and NAA for tissue culture and rapid propagation were studied.The results showed that the 1/2MS medium with sucrose 30 g/L,BA 2.0 mg/L and NAA 0.2 mg/ L was the best for multiplication culture.The 1/4MS medium with sucrose 15 g/L,IBA 1.5 mg /L and NAA 0.5 mg/L was the best treatment for inducing roots from shoots,and the rootage ratio was 92%. |
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| Keywords: | Nepenthes mirabilis tissue culture multiplication root |
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