河北省小麦品种高分子量谷蛋白亚基组成分析

高分子量麦谷蛋白(HMW-GS)亚基组成是决定小麦加工品质的重要蛋白组分,对小麦品质具有重要影响。本研究利用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)方法分析了148份河北省小麦品种的高分子量麦谷蛋白亚基(HMW-GS)组成及其历史变化。结果表明:在148份小麦品种中共检测到16种HMW-GS亚基和43种亚基组合。在Glu-A1位点,有3种亚基类型,以null和1亚基为主;Glu-B1位点有7种亚基类型,以"7+9"和"7+8"亚基为主;Glu-D1位点有6种亚基类型,以"2+12"亚基类型为主。在43种亚基组合中,以"null、7+9、2+12"组合为主,其他亚基组合尤其是与品质有关的亚基组合相对较少。从河北省小麦品种HMW-GS的历史演变来看,优质蛋白亚基及其组合虽然近年来有所提高,但仍需进一步加强。

Analysis of high molecular weight glutenin subunits composition of bread wheat cultivars released in Hebei province from 1949 to 2008

Subunit compositon of high molecular weight glutenins(HMW-GS) in seed kernels is of great importance for wheat quality improvements.In this study,one hundred and forty-eight accessions of bread wheat(Triticum aestivum L.) released during 1965-2008 in Hebei,China,were analyzed with SDS-PAGE methods.The results showed that a total of 16 HMW-GS subunits and 43 subunit combinations were detected in the cultivars studied.Of the 16 subunits,null,'7+9' and '2+12' were predominant types encoded by genes located in Glu-A1,Glu-B1 and Glu-D1,respectively.Of the 43 HMW-GS subunit combinations,'null/7+9/2+12' occurred predominantly.HMW-GS subunits and corresponding combinations for good quality were poor in the cultivars analyzed.Though the historical changes of HMW-GS in Hebei wheat cultivars implied frequencies of some "good subunits" and their combinations increased to some extent,enancement of such "good subunits" in future breeding programs need to be strenthened.