激素对杜仲幼茎愈伤组织诱导及生长的影响

以MS为基本培养基,添加不同浓度的2,4-D,NAA,IBA,BA,KT或其组合,研究杜仲嫩茎愈伤组织的诱导及生长情况。结果表明,3种生长素均可100%诱导出愈伤组织,以0.5mg/L2,4-D,0.5～1.0mg/LNAA,1.0mg/LIBA效果较好;2,4-D和BA及KT的组合均不利于愈伤组织诱导,低浓度的BA与NAA和IBA组合能促进愈伤组织的诱导和生长,其中1.0mg/LNAA+0.3～0.5mg/LBA,1.0mg/LIBA+0.5mg/LBA诱导出的愈伤组织生长旺盛,富有光泽,适合进一步继代培养,而高浓度则起抑制作用;KT的加入对愈伤组织的诱导及生长均起抑制作用。

Effect of phytohormomones on shoot callus induction and growth of Eucommia ulmoides Oliv

The shoots of Eucommia ulmoides Oliv.as explants,the MS as the basic madium supplemented with 2,4-D,NAA,IBA,KT,the callus induction and growth of E.ulmoides Oliv.were studied.The results showed that,calluses were induced 100 per cent when explants were cultured on MS medium supplemented with three auxins.0.5 mg/L 2,4-D,0.5-1.0 mg/L NAA,1.0 mg/L IBA was best for callus induction and growth.Combination of 2,4-D and BA or KT was worse than 2,4-D singly.Low concentrated BA and NAA or IBA combinations had better effect than singly,but high concentrated BA was bad.The best combination of callus induction and growth was 1.0 mg/L NAA+0.3-0.5 mg/L BA or 1.0 mg/L IBA+0.5 mg/L BA,whose calluses were growing quickly,glossy,suitable for subculture.KT was bad for callus induction and growth.