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| 磁性微粒在核酸研究中的应用 | |
| 引用本文： | 崔亚丽,李 铮,房 喻.磁性微粒在核酸研究中的应用[J].西北农林科技大学学报(社会科学版),2000,28(1):80-85. |
| 作者姓名： | 崔亚丽 李 铮 房 喻 |
| 作者单位： | 1. 西安交通大学化工学院生物科学与工程系,西安,710049;陕西师范大学化学系,西安,710062 2. 西安交通大学化工学院生物科学与工程系,西安,710049 3. 陕西师范大学化学系,西安,710062 |
| 摘 要： | 磁性微粒作为固相载体应用于生物分子的合成、分离和检测,是一种非常有效、快速和方便的方法。在挪威、英、美、德等国已实现了商业化并得到了广泛的应用,而我国目前还处于研究阶段。对磁性微粒在mRNA的分离与纯化、DNA的分离与纯化以及核酸的杂交、扩增、检测等核酸研究领域中的应用进行了较为全面的综述。 |
| 关 键 词： | 磁性微粒 固相载体 核酸研究 |
| 文章编号： | 1000-2782(2000)01-0080-06 |
| 修稿时间： | 1999-07-14 |
The application of magnetic particles used in nucleic acid research |
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| CUI Ya li \{,\},LI Zheng,FANG Yu,ZHAO Wen ming.The application of magnetic particles used in nucleic acid research[J].Journal of Northwest Sci-Tech Univ of Agr and,2000,28(1):80-85. | |
| Authors: | CUI Ya li \{ \} LI Zheng FANG Yu ZHAO Wen ming |
| Institution: | 1 (1 Department of Bioscience and Engineering,Xian Jiaotong University,Xian 710049,China) (2 Department of Chemisty,Shaanxi Normal University,Xian 710062,China) |
| Abstract: | As solid carrier,magnetic particles have proven to be very efficient,rapid and convenient for synthesis,separation and detection of biomolecules.It has already been commercialized in Norway,England,America and Germany and used in many aspects in molecular biology.It is still in researching stage in China.In this review,magnetic particles for the purification of mRNA and DNA,for the hybridization and detection of mRNA and DNA,additionally in other areas such as DNA sequencing,the amplification of DNA and the construction of cDNA library will be covered. |
| Keywords: | magnetic particle solid carrier nucleic acid research |
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