蝴蝶兰高效离体繁殖途径的研究

以蝴蝶兰花梗为初次诱导的外植体,对蝴蝶兰的离体繁殖途径进行了研究。结果发现,通过花梗腋 芽萌发可获得无菌芽,再以所得的嫩芽茎段和叶子作为离体培养的增殖材料,2-3个月后便可获得大量的不定芽。 该结果表明:(1)在初次培养中加入PVP并进行暗培养,其抗褐化效果最佳。(2)增殖培养基以MS+6-BA 0．5 mg／L+KT 1．0 mg／L+NAA 0．2 mg／L或MS+6-BA 2．0 mg／L+NAA 0．5 mg／L组合较好,其茎段萌发率均高 达90％,增殖倍数分别高达2．8和2．9;叶片芽的诱导率分别为56％和57％,增殖倍数最高达4．8和4．6。(3)经济 有效的壮苗和生根诱导培养基为“改良VW+6-BA 0．1 mg／L+NAA 0．5 mg／L+IBA 0．2 mg／L”,芽的有效率为 88％,发根率达93％。

A high efficient protocol for Phalaenopsis in vitro propagation

A high efficient protocol for Phalaenopsis in vitro propagation was described with pedicels as explants for initial culture. Results showed that the shoots could be produced by axillaries budding from explants. And using young stems and leaves as multiplication material in vitro culture,regeneration shoots were obtained directly approximately 2 - 3 months after culture. The results indicated that: (1) the optimum method for browning resistance was media supplement with PVP and under darkness for initial culture. (2) The optimum media for multiplication was:MS + 6-BA 0. 5 mg/L + KT 1. 0 mg/L + NAA 0. 2 mg/L or MS+6-BA 2. 0 mg/L + NAA 0. 5 mg/L. For stem,both of the germination rate went up to 90%; and one multiplication time was up to 2. 8,the other 2. 9. For leaf,one of the induction rates was 56% ,the other 57%;and one multiplication time reached 4. 8,the other 4. 6. (3) An efficient and economic medium for elongation and rooting was modified VW + 6-BA 0. 1 mg/L + NAA 0. 5 mg/L + IBA 0. 2 mg/L,the effective rate of shoot was 88% and the rate of rooting was 93%.