提高N6培养基钙浓度对玉米幼胚培养的影响

筛选试验结果表明 ,将 N6 培养基 Ca2 + 浓度从 1.13mmol/ L提高到 5 m mol/ L ,对玉米幼胚胚性愈伤组织的诱导、继代和分化具有促进作用。在一次继代过程中 ,Ca2 +浓度为 5 m mol/ L 的培养基上培养的胚性愈伤组织相对生长量比 1.13m mol/ L 的原培养基增加 0 .8倍。 Ca2 +浓度提高以后 ,在培养基中再添加钙调蛋白 (Ca M)抑制剂盐酸氯丙嗪 (CPZ) ,可抑制 Ca2 +的促进作用 ,说明钙增加后对玉米胚性愈伤组织诱导和继代的促进作用受Ca2 + / Ca M信息系统控制。用改进后的 N6 培养基 ,可提高生产上大面积推广的玉米自交系胚性愈伤组织的诱导率和继代生长量

The effect of raising calcium consistency of N6 medium on maize immature embryos'culture

The selection experiment showed that improved N 6 medium by increasing Ca 2+ content from 1.13 to 5 mmol/L promoted embryogenic callus induction and subculture growth from maize immature embryos.During one time of subculture,growth rate of embryogenic callus increased by 0.8 times as subcultured on the medium with Ca 2+ content of 5 mmol/L as subcultured on the medium with Ca 2+ content of \{1.13\} mmol/L.Addition of CPZ,CaM inhibitor,to the medium with 5 mmol/L Ca 2+ performed an inhibition effect on the promotion role of Ca 2+ .The improved N 6 medium could be used to increase embryogenic callus induction rate and subculture growth rate from popularized maize inbreds and provide acceptor system with excellent genetic foundation for transgenic operation.