| 培养条件对马铃薯晚疫病菌粗毒素产生的影响 |
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| 引用本文： | 邢宇俊,程智慧.培养条件对马铃薯晚疫病菌粗毒素产生的影响[J].西北农林科技大学学报(社会科学版),2006,34(3):89-92. |
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| 作者姓名： | 邢宇俊 程智慧 |
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| 作者单位： | 西北农林科技大学,园艺学院,陕西,杨凌,712100 |
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| 基金项目： | 中-德合作项目;中-德合作项目 |
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| 摘 要： | 研究了马铃薯晚疫病病原菌菌株5-4在不同培养基、光照、温度、pH值、振荡培养方式等条件下产生毒素的情况,并用番茄种子发芽法检测了毒素的毒性。结果表明,适宜马铃薯晚疫病菌粗毒素培养的培养基为黑麦液体培养基,培养基pH值为6．0-7．0,培养温度20-22 C,在黑暗条件下连续振荡培养16 d时产生的粗毒素的毒性最强。
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| 关 键 词： | 马铃薯晚疫病菌 培养条件 粗毒素 |
| 文章编号： | 1671-9387(2006)03-0089-04 |
| 收稿时间： | 2005-07-21 |
| 修稿时间： | 2005年7月21日 |
The effects of culture conditions on production of Phytophthora infestans crude toxin |
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| XING Yu-jun,CHENG Zhi-hui.The effects of culture conditions on production of Phytophthora infestans crude toxin[J].Journal of Northwest Sci-Tech Univ of Agr and,2006,34(3):89-92. |
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| Authors: | XING Yu-jun CHENG Zhi-hui |
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| Institution: | (College of Horticulture,Northwest A & F University ,YanglingtShaanxi 712100,China) |
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| Abstract: | The effects of pathogen Phytophthora infestans culture conditions,such as culture medium, light .temperature ,pH value and shaking,on crude toxin production were studied using tomato seed germination as the biologic test of toxicity of crude toxin. The results showed that the favorable culture conditions were rye liquid medium,pH value 6. 0-7. 0,culture temperature 20-22 C with successive darkening and shaking during culture. The production of crude toxin reached the highest level on the 16th day of culture. |
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| Keywords: | Phytophthora infestans culture condition crude toxin |
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