板蓝根、大青叶中靛蓝和靛玉红的测定方法比较

为了筛选出合适的测定板蓝根、大青叶中靛蓝、靛玉红含量的方法,对比、分析了高效液相色谱法、双波长分光光度法和薄层扫描法测定的稳定性、精密度和加标回收率等。结果表明,高效液相色谱法测定靛蓝、靛玉红含量的各项指标均优于其他方法;双波长分光光度法由于无法排除提取液中其他成分对靛蓝、靛玉红的干扰,使得测定结果偏高;薄层扫描法不适宜于板蓝根中靛蓝、靛玉红含量的测定,而可用于大青叶中这2种成分的定量,但测定必须在薄层板展开后3 h内完成。可见3种分析方法各有优缺点,可用于不同精度下对靛蓝、靛玉红含量的测定。

Comparison of three methods of measuring indigo and indirubin in radix isatidis and folium isatidis

In order to obtain the suitable measure to select indigo and indirubin contained in radix isatidis and folium isatidis,three methods i.e.,HPLC,DWSP and TLCS were compared from the aspects of stability,precision and recovery.The results are as follows:The HPLC is the best one.The DWSP makes the results higher than true due to the interference of the impurities.The TLCS is not suitable to measure the contents of indigo and indirubin in radix isatidis,but suitable in folium isatidis if the test can be accomplished within three hours after the development of two constituents.Each of the three methods has its advantages and disadvantages and can be adopted in different precisions.