猪胚胎细胞核移植

选用杜洛克２～１６细胞期胚胎卵裂球作核供体，湖北白猪卵母细胞作核受体，通过显微操作和电融合法构成重组胚。体外培养时，以融合前２ｈ，１ｈ激活及融合前不激活的卵母细胞做核受体的重组胚，发育率分别为６４．６％（３１／４８），５５．３％（２６／４７）和３４．５％（１９／５５）。６１枚重组胚移入同步发情的５头受体母猪输卵管，１头于妊娠１１７ｄ产下５头核移植仔猪。结果表明，激活卵母细胞作核受体优于未激活卵母细胞。成熟卵母细胞的胞质对于移入的核具有重排能力

Nuclear Transplantation in Porcine Embryos

Durac was used as nuclear donor and Hubei White used as nuclear recipient.Reconstructive embryos were produced with electrofused method by micromanuplantation.Thedevelopmentrateofnuclear transfer embryos (NTE) was 64.6%,55.3% and 34.5% ( P <0.05) respectively,when the recipient oocytes were activated two hours,one hour prior to fusion and nonactivated.61 nuclear transferred reconstructive embryos were transferred to five recipient sows.One recipient calved five nuclear transfer piglets.The resultes indicated that activated oocytes could be used as recipient and could remodel the transferred nucleus.The birth of NT piglets showed that the technical parameters and procedures in this paper were suitable for nuclear transplantation in porcine embryos.