高温胁迫对猕猴桃幼苗叶片某些生理效应的影响

在45℃下对猕猴桃一年生盆栽苗进行2,4,6,8,10h的高温胁迫,测定高温胁迫下叶绿素含量、相对膜透性、丙二醛(MDA)含量、抗氧化酶活性和抗氧化剂含量及热激蛋白等生理生化指标。结果表明,高温胁迫下叶绿素含量下降,相对膜透性增大,MDA含量增加;超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)、抗坏血酸还原酶(APX)活性均在胁迫初期呈上升趋势,随处理时间延长,活性下降;随胁迫时间延长,还原型谷胱甘肽(GSH)含量先升后降,抗坏血酸(ASA)含量总体呈下降趋势,谷胱甘肽还原酶(GR)和脯氨酸含量一直呈上升趋势;热激蛋白(HSP)在高温下被诱导。

Physiological effects of kiwifruit lamina under high temperature stress

The kiwifruit was treated with different time period of high temperature stress,and the physiological index changes in leaves of kiwifruit were determined,such as the content of chlorophyll,leakage of electrolyte,content of MDA,activities of antioxidant enzymes,content of endogenous antioxidants and heat shock protein under high temperature.The results indicated that under high temperature stress,the content of chlorophyll decreased,and leakage of electrolytes and content of MDA increased.The activities of antioxidant enzymes (SOD,POD,CAT,APX) increased under first period of temperature stress ((0-4) hour),and then decreased.The content of ASA was decreased,GSH increased from 0 to 4 hours,decreased from 4 to 10 hours,GR and proline content increased during all the period.Therefore,it suggested that kiwifruit was injured under high temperature.Meanwhile,the protecting and adaptive mechanisms of plant were initiated to protect plant under high temperature stress.