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玉米耐盐愈伤组织变异体的筛选及耐盐性分析
引用本文:韦小敏,何金环,陈彦惠.玉米耐盐愈伤组织变异体的筛选及耐盐性分析[J].西北农林科技大学学报(社会科学版),2007,35(9):73-78.
作者姓名:韦小敏  何金环  陈彦惠
作者单位:1. 郑州牧业工程高等专科学校,生物工程系,河南,郑州,450011;河南农业大学,农学院,河南,郑州,450002
2. 郑州牧业工程高等专科学校,生物工程系,河南,郑州,450011
3. 河南农业大学,农学院,河南,郑州,450002
摘    要:为了筛选玉米耐盐愈伤组织变异体,选用4个适于玉米组织培养基因型的幼胚为外植体,诱导出胚性愈伤组织。继代5次后,分别用200 mmol/L NaCl和5.5 mmol/L Hyp连续筛选3次,获得耐盐愈伤组织变异体,分别称为rn变异体和rh变异体,以普通继代培养基上培养的愈伤组织为对照。经一代回复培养,分别在200,250和300 mmol/L NaCl胁迫下,分别测定了rn变异体r、h变异体和对照愈伤组织存活率,胚性愈伤组织百分率,K+、Na+含量;在300 mmol/L NaCl胁迫下,测定了rn变异体、rh变异体和对照愈伤组织的生长量及脯氨酸含量。结果表明,rh变异体愈伤组织的分化频率高于rn变异体,Hyp的选择频率是NaCl的2倍左右;在不同浓度NaCl胁迫下,rh变异体具有较高的愈伤组织存活率和K+、Na+含量;在300 mmol/L NaCl胁迫下,rh变异体的生长量和脯氨酸含量均最高,表现出了较好的耐盐性。

关 键 词:玉米  耐盐愈伤组织变异体  植物耐盐性
文章编号:1671-9387(2007)09-0073-06
收稿时间:2006/8/31 0:00:00
修稿时间:2006-08-31

Selection of salt-tolerant callus variants in maize and analysis on salt-tolerance
WEI Xiao-min,HE Jin-huan,CHEN Yan-hui,JI Liang-yue.Selection of salt-tolerant callus variants in maize and analysis on salt-tolerance[J].Journal of Northwest Sci-Tech Univ of Agr and,2007,35(9):73-78.
Authors:WEI Xiao-min  HE Jin-huan  CHEN Yan-hui  JI Liang-yue
Institution:1 Department of Biology,Zhengzhou Animal Husbandry Engineering College,Zhengzhou,He’nan 450011,China;2 College of Agronomy,He’nan Agricultural University,Zhengzhou He’nan 450002,China)
Abstract:In order to obtain salt-tolerant callus variants in maize,the embryogenic calli were induced from immature embryos of 4 maize genotypes that were suitable for tissue culture.After 5 generations culture,salt-tolerant calli variants were obtained through three continuous selection with 200 mmol/L NaCl and 5.5 mmol/L Hyp,called rn variant and rh variant respectively,at the same time,callus from basal subculture medium was used as CK.Through one generation back-culture,under 200,250 and 300 mmol/L NaCl stress,callus survival ratio,embryogenic callus proportion and K and,Na contents of rn variant,rh variant and CK were counted or tested;growth quantity and free proline content of rn variant,rh variant and CK were tested under 300 mmol/L NaCl stress.The results showed that the differentiation frequency of rh variant was higher than that of rn variant;the selection frequency of Hyp was twice as much as that of NaCl.The rh variant had higher callus survival ratio and the contents of K and Na under different concentration salt stress;growth quantity and free proline content of rh variant under 300 mmol/L NaCl stress were the largest,showing stronger salt-tolerance.
Keywords:maize  salt-tolerant callus variants  plant salt-tolerance
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