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凉山半细毛羊、山谷型藏绵羊染色体G-带、C-带核型研究
引用本文:李小勤,吴登俊,陈圣偶.凉山半细毛羊、山谷型藏绵羊染色体G-带、C-带核型研究[J].西北农林科技大学学报(社会科学版),2007,35(1):6-12.
作者姓名:李小勤  吴登俊  陈圣偶
作者单位:1. 上海水产大学,生命科学与技术学院,上海,200090
2. 四川农业大学,动物科技学院,四川,雅安,625014
基金项目:德国联邦教育与研究部(BMBF)资助项目;上海市重点学科建设项目
摘    要:以凉山半细毛羊、山谷型藏绵羊为研究对象,以微量全血培养法制备染色体标本,采用GTG法和CBG法获得染色体G-带和C-带,并对其核型进行分析。结果表明,两品种羊的G-带核型基本一致;以凉山半细毛羊和山谷型藏绵羊G-带核型为基础所作的G-带核型模式图共有275条带,其中阳性带129条;两品种羊C-带核型在分布上无明显差异。

关 键 词:凉山半细毛羊  山谷型藏绵羊  染色体核型  G-带核型  C-带核型  G-带模式图
文章编号:1670-9387(2007)01-0006-07
收稿时间:2006/6/28 0:00:00
修稿时间:2006-06-28

Comparison study on the G-banded and C-banded karyotypes in Liangshan semifine-wool sheep and Tibetan sheep
LI Xiao-qin,WU Deng-jun,CHEN Sheng-ou,LENG Xiang-jun.Comparison study on the G-banded and C-banded karyotypes in Liangshan semifine-wool sheep and Tibetan sheep[J].Journal of Northwest Sci-Tech Univ of Agr and,2007,35(1):6-12.
Authors:LI Xiao-qin  WU Deng-jun  CHEN Sheng-ou  LENG Xiang-jun
Institution:1 Shanghai Fisheries University, Shanghai 200090,China ; 2 College of Animal Science and Technology,Sichuan Agricultural University,Yaan,Sichuan 625014 ,China
Abstract:Four Liangshan semifine-wool sheep and four Tibetan sheep were used to compare the G-banded and C-banded karyotypes by GTG,CBG techniques,which were employed on slides obtained from the peripheral blood cell culture.The result showed that:the two breeds had the same G-banding patterns.According to the Liangshan semifine-wool sheep's and Tibetan sheep's G-bands,G-banding ideogram was described.There were 275 bands including 129 positive bands.There was no distinguished difference in C-banding patterns of the two breeds,but the C-banding pattern of the Liangshan semifine-wool sheep was lighter and smaller than that of Tibetan sheep's.
Keywords:Liangshan semifine-wool sheep  Tibetan sheep  chromosome karyotype  G-banded karyotype  C-banded karyotype  G-banded ideogram
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