脱毒马铃薯感染青枯病后根部的生理变化

用肿囊腐霉的孢子悬液分别对脱毒马铃薯抗病自交系 F1 和感病自交系 F的扦插苗进行接种 ,并测定了接种后根中苯丙氨酸解氨酶 (PAL)、过氧化物酶 (POD)、超氧化物歧化酶 (SOD)的活性和可溶性蛋白的含量。结果表明 ,接种后 ,脱毒马铃薯抗病自交系 F1 的 PAL 活性曲线呈现 2个峰 ,而感病自交系 F的 PAL 活性曲线呈现 1个峰 ;病原菌感染早期 ,F1 ,F的 POD和 SOD活性都有不同程度的下降 ,但到后期 ,F1 的 POD和 SOD活性均迅速回升 ,而 F的则继续维持低水平 ;F1 的可溶性蛋白含量在接种后呈现 1个峰 ,到后期维持较高含量 ,而 F的可溶性蛋白含量持续减少。

Study on the physiological changes of dehydrated virus potato root during infection with Pythium inflatum

Dynamic changes in activities of phenylalanine ammonialyase,peroxidase,superoxide dismutases and soluble protein contents were analysed during infection by pythium inflatum in resistant inbred line F 1 and susceptible inbred line F of dehydrated virus potato.After inoculation,the activities of the PAL of F 1 showed two peaks,while that of F showed only one peak.In initial stage of penetration by the fungus,POD and SOD activities in both resistant and susceptible inbred lines showed a trend of decrease,while in late stage of the infection F 1 displayed greater increase of POD and SOD.But the activity of both enzymes in F still stayed in a lower level.In initial stage of infection,soluble protein contents of F 1 showed one peak and become significantly higher on the contrary.But that of F showed a trend of decrease.