| EMS诱导小麦条锈菌毒性突变的研究 |
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| 引用本文： | 姚秋燕,王国芬,徐智斌.EMS诱导小麦条锈菌毒性突变的研究[J].西北农林科技大学学报(社会科学版),2006,34(6):120-123. |
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| 作者姓名： | 姚秋燕 王国芬 徐智斌 |
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| 作者单位： | 西北农林科技大学,植保学院,陕西省农业分子生物学重点实验室,陕西,杨凌,712100 |
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| 基金项目： | 陕西省科技攻关项目;教育部长江学者和创新团队发展计划 |
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| 摘 要： | 以化学诱变剂EM S处理小麦条锈菌CY 17和CY 31的夏孢子诱发其毒性突变,用抗条锈Y r近等基因系筛选毒性突变体,建立了4个CY 17和1个CY 31的突变菌株,各筛选品种上的病菌突变频率明显不同,突变率在2.14×1-0 6~3.68×1-0 5。对突变菌株的毒性测定结果表明,CY 17的所有突变菌株对Y r12发生了毒性突变,且CY 17和CY 31的所有突变菌株对Y r5,Y r10,Y r15和Y r24等4个抗病基因表现无毒性。研究证实毒性突变是小麦条锈病菌毒性变异的重要途径。
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| 关 键 词： | 小麦条锈菌 EMS诱导 毒性突变 |
| 文章编号： | 1671-9387(2006)06-0120-04 |
| 收稿时间： | 2005-12-26 |
| 修稿时间： | 2005年12月26 |
Virulent mutant in Puccinia striiformis induced by ethyl methyl sulfomar(EMS) |
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| YAO Qiu-yan,WANG Guo-fen,XU Zhi-bin,WANG Mei-nan,WANG Yang,JING Jin-xue.Virulent mutant in Puccinia striiformis induced by ethyl methyl sulfomar(EMS)[J].Journal of Northwest Sci-Tech Univ of Agr and,2006,34(6):120-123. |
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| Authors: | YAO Qiu-yan WANG Guo-fen XU Zhi-bin WANG Mei-nan WANG Yang JING Jin-xue |
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| Institution: | (College of Plant Protection,Shaanxi Key Laboratory of Molecular Biology for Agriculture, Northwest A & F University,Yang ling,Shaanxi 712100,China) |
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| Abstract: | Urediospores of CY17 and CY31 races of Puccinia striiformis were treated by ethyl methane sulfonate (EMS) and virulent mutants were screened on stripe rust resistant near isogenic lines inoculated with treated spores.4 virulent and avirulent mutants from CY17 and 1 virulent mutants from CY31 were established.Mutation rate ranged between 2.14×10-6-3.68×10-5.Results indicated that mutants of CY17 were virulent to Yr12,but all mutants of CY17 and CY31 were avirulent to Yr5,Yr10,Yr15 and Yr24.The results suggested that mutation was important mechanism of virulence variation in rust fungi. |
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| Keywords: | Puccinia striiform ethyl methane sulfonate(EMS) induction virulent mutation |
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