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| 植物afp表达载体的构建及其对农杆菌的直接转化 | |
| 引用本文： | 尹明安,崔鸿文,樊代明.植物afp表达载体的构建及其对农杆菌的直接转化[J].西北农林科技大学学报(社会科学版),2001,29(2):1-5. |
| 作者姓名： | 尹明安 崔鸿文 樊代明 |
| 作者单位： | 1. 西北农林科技大学园艺学院, 2. 第四军医大学西京医院, 3. 西北农林科技大学生命科学学院, |
| 基金项目： | 陕西省科技攻关项目（98K12-G19（11）） |
| 摘 要： | 详细报道了胡萝卜抗冻蛋白基因 (afp)植物表达载体的构建过程及其直接法转化农杆菌的方法。以限制性内切酶从克隆载体上切取 afp基因 ,将其连接在相应酶切的 p BI12 1工程质粒上 ,构建成 afp植物表达载体。采用直接转化法将重组子导入根癌农杆菌 ,并用 PCR方法进行了鉴定 |
| 关 键 词： | 植物抗冻蛋白 农杆菌 分子生物学 胡萝卜 |
| 文章编号： | 1000-2782(2001)02-001-05 |
| 收稿时间： | 9/1/2000 12:00:00 AM |
| 修稿时间： | 2000年9月1日 |
Construction of plant afp expression vector and its direct transformation to agrobacterium |
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| YIN Ming-an,CUI Hong-wen,FAN Dai-ming,GUO Li.Construction of plant afp expression vector and its direct transformation to agrobacterium[J].Journal of Northwest Sci-Tech Univ of Agr and,2001,29(2):1-5. | |
| Authors: | YIN Ming-an CUI Hong-wen FAN Dai-ming GUO Li |
| Institution: | YIN Ming- an1,CUI Hong- wen1,FAN Dai- ming2,GUO Li1 |
| Abstract: | Construction of a vector expressing carrot antifreeze protein gene(afp) in plants and its direct transformation to agrobacterium were thoroughly reported.Clone vector was digested with restriction endonucleases,and the acquired afp was ligated to engineered plasmid p BI1 2 1 which was digested with appropriate restriction endonucleases,and therefore a plant expression vector for carrot afp was constructed.The recombinant was transferred into agrobacterium tumefacien by direct DNA transfer and the transformant was identified with PCR. |
| Keywords: | plantantifreeze protein agrobacterium molecular biology carrot |
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