海南菜豆上粉虱传双生病毒的分子检测

为了解粉虱传双生病毒在海南的发生情况,从海南儋州采集了3个菜豆病样,症状表现为花叶、皱缩、叶背部叶脉突起,根据粉虱传双生病毒基因组DNA基因间隔区及外壳蛋白基因保守序列设计的简并引物,对这3个样品进行了PCR检测,从其中两个样品中扩增到预期大小为522 bp的DNA片段。PCR产物经克隆后进行序列测定,BLAST分析结果表明,该序列与与印度绿豆黄花叶病毒豇豆分离物(GenBank登录号:AF481865)的同源性最高,为65.9%,说明海南菜豆中存在着粉虱传双生病毒的侵染。

Molecular detection of whitefly-transmitted Geminivirus in Phaseolus vulgaris L. in Hainan Island

To investigate the occurrence of whiteflytransmitted geminivirus in Phaseolus vulgaris L.in Hainan,three virus-infected Phaseolus vulgaris L.samples with mosaic,crinkle and vein plum symptom were collected in Danzhou,Hainan Province.A degenerate primer pair was designed according to the conserved sequence of CP gene and intergenic spacer of WTG,and a single PCR product of expected size(522 bp) was amplified from two samples by PCR,cloned and sequenced.BLAST analysis showed that PCR product had a geminiviral gene origin,and the sequence had the highest sequence identity(65.9%) with that of Mungbean yellow mosaic India virus-Cowpea](AF481865),which indicated that two samples were infected with geminiviruses.