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大赖草和新麦草物种专化DNA重复序列研究Ⅱ在小麦族中分布的多态性
引用本文:柴守诚,刘大钧,陈佩度.大赖草和新麦草物种专化DNA重复序列研究Ⅱ在小麦族中分布的多态性[J].西北农林科技大学学报(社会科学版),1999,27(4):1-6.
作者姓名:柴守诚  刘大钧  陈佩度
作者单位:1. 西北农业大学农学系,陕西杨陵,712100
2. 南京农业大学农业部作物细胞遗传重点开放实验室,南京,210095
摘    要:大赖草和新麦草中克隆的5个DNA重复序列与小麦族不同物种DNA进行Southern杂交。结果表明,这5个重复序列在Leymus属(赖草属)和Psathyrostachys属(新麦草属)中的丰度与小麦、大麦和黑麦相差悬殊,可以作为这2个属染色体的分子标记检测其导入小麦、大麦和黑麦后的踪迹,尤其是pLr647对这2个属专化性更强,应优先选用。另外,结合本研究结果对小麦族有关种属染色体组的进化问题进行了讨论。

关 键 词:大赖草  新麦草  小麦族  DNA重复序列
修稿时间:1998-12-29

A Study on Species-Specific DNA Repeats from Leymus racemosus and Psathyrostachys juncea Ⅱ Polymorphism among the Species of Triticea
CHAI Shou-cheng,LIU Da-jun,CHEN Pei-du,LI Wan-long,QI Li-li,CAO Ming-shu,LIU Jing-yuan.A Study on Species-Specific DNA Repeats from Leymus racemosus and Psathyrostachys juncea Ⅱ Polymorphism among the Species of Triticea[J].Journal of Northwest Sci-Tech Univ of Agr and,1999,27(4):1-6.
Authors:CHAI Shou-cheng  LIU Da-jun  CHEN Pei-du  LI Wan-long  QI Li-li  CAO Ming-shu  LIU Jing-yuan
Institution:CHAI Shou cheng,LIU Da jun,CHEN Pei du,LI Wan long, QI Li li,CAO Ming shu,LIU Jing yuan (Key Laboratory of Crop Cytogenetics of the Ministry of Agriculture, Nanjing Agricultural University,Jiangsu,Nanjing 210095,China)
Abstract:The abundance and RFLP of 5 DNA repeats,pLr344,pLr647,pLr426,pPj205 and pPj605 appearing in various species of tribe Triticeae were tested by Southern blot hybridization.The big differences among species of Triticeae in abundance of the five DNA repeats made it possible that they could be used as molecular markers to monitor Leymus and Psathyrostachys chromatins transferred to wheat,barley and rye.Owing to being more strictly species specific,pLr647 was particularly recommended for this purpose.The evolution of some genera in wheat tribe was also discussed.
Keywords:Triticeae  Leymus racemosus  Psathyrostachys juncea  repeated DNA sequence  
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