| 鲤肠黄脉病样中烟粉虱传双生病毒的检测 |
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| 引用本文： | 何自福,虞皓,毛明杰,佘小漫.鲤肠黄脉病样中烟粉虱传双生病毒的检测[J].西北农林科技大学学报(社会科学版),2005,33(Z1):137-139. |
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| 作者姓名： | 何自福 虞皓 毛明杰 佘小漫 |
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| 作者单位： | 广东省农业科学院植物保护研究所,广东省植物保护新技术重点实验室,广东,广州,510640 |
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| 基金项目： | 广东省自然科学基金(031992);广东省重点实验室二期建设启动项目(2003B60128) |
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| 摘 要： | 在严重发生番茄曲叶病的田间,发现主要杂草鲤肠表现出叶脉褪绿黄化、叶片皱缩或卷曲等症状,田间病株率达63%;用烟粉虱传双生病毒兼并引物对随机采集的20个鲤肠病样进行PCR检测,结果发现,从这些病样中均能扩增出1条356 bp的特异片段;室内人工接种试验结果显示,鲤肠分离物可以侵染番茄而引起曲叶症状。因此,鲤肠可能是广东番茄曲叶病毒的自然中间寄主。
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| 关 键 词： | 鲤肠黄脉病 烟粉虱传双生病毒 PCR检测 |
| 文章编号： | 1671-9387(2005)S0-0137-03 |
| 修稿时间： | 2005年7月8日 |
Eclipta prostrata yellow vein disease caused by whitefly-transmitted geminiviruses |
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| HE Zi-fu,YU Hao,Mao Ming-jie,SHE Xiao-man.Eclipta prostrata yellow vein disease caused by whitefly-transmitted geminiviruses[J].Journal of Northwest Sci-Tech Univ of Agr and,2005,33(Z1):137-139. |
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| Authors: | HE Zi-fu YU Hao Mao Ming-jie SHE Xiao-man |
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| Institution: | (Institute of Plant Protection,Guangdong Academy of Agricultural Sciences,Guangzhou,Guangdong 510640,China) |
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| Abstract: | Eclipta prostrata showed yellow vein,crimple leaf and curl leaf in the fields,where the tomato plants infected by tomato leaf curl Guangdong virus.The diseased incidence of Eclipta prostrata was 63% in the fields.Twenty diseased samples,collected randomly from the fields,were detected by PCR.The results showed that the specific 356 bp-fragment was amplified from all of the samples.The results of artificially inoculated test showed that the isolate of Eclipta prostrata yellow vein virus caused tomato leaf curl symptom.These results proved that Eclipta prostrata probably was the natural medi-host plant of ToLCGDV. |
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| Keywords: | Eclipta prostrata yellow vein disease whitefly-transmitted geminiviruses PCR detection |
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