| 富士苹果黄化茎段再生不定芽的研究 |
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| 引用本文： | 王贵章,徐凌飞,马锋旺.富士苹果黄化茎段再生不定芽的研究[J].西北农林科技大学学报(社会科学版),2006,34(6):79-81. |
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| 作者姓名： | 王贵章 徐凌飞 马锋旺 |
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| 作者单位： | 西北农林科技大学,园艺学院,陕西,杨凌,712100 |
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| 基金项目： | 教育部优秀青年教师资助计划;西北农林科技大学校科研和教改项目 |
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| 摘 要： | 以苹果主栽品种长富2号为试材,研究了不同培养基及6-BA和NAA浓度对黄化且不带腋芽的节间茎段再生效率的影响。结果表明,3个因子对长富2号黄化茎段再生的影响程度依次为基本培养基>6-BA>NAA;最适培养基为M S+6-BA 5.0 m g/L+NAA 0.4 m g/L+CH 200 m g/L+蔗糖30 g/L+琼脂7 g/L,再生频率可达83%,最高平均再生芽数为3.03。
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| 关 键 词： | 富士苹果 长富2号 黄化茎段 再生体系 |
| 文章编号： | 1671-9387(2006)06-0079-03 |
| 收稿时间： | 2005-08-31 |
| 修稿时间： | 2005年8月31日 |
Adventitious buds regeneration from etiolated internode segment of apple cultivar Nagafu No.2 |
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| WANG Gui-zhang,XU Ling-fei,MA Feng-wang,LIANG Dong.Adventitious buds regeneration from etiolated internode segment of apple cultivar Nagafu No.2[J].Journal of Northwest Sci-Tech Univ of Agr and,2006,34(6):79-81. |
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| Authors: | WANG Gui-zhang XU Ling-fei MA Feng-wang LIANG Dong |
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| Institution: | (College of Horticulture,Northwest A & F University,Yangling,Shaanxi 712100,China) |
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| Abstract: | The effects of different basal medium,level of 6-BA and NAA on adventitious bud regeneration from etiolated intermode segment of Nagafu No.2 were studied by using the orthogonal experiment design.The results showed that the effective order of three factors on regeneration was basal medium,6-BA and NAA.The optimum culture medium was 6-BA 5.0 mg/L+NAA 0.4 mg/L+CH 200 mg/L+Sucrose 30 g/L+Agar 7.0 g/L;and on this medium,the regeneration frequency and mean number of bud per segment were 83% and 3.03 respectively. |
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| Keywords: | Fuji apple Nagafu No 2 etiolated internode segment regeneration system |
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