番茄叶霉病拮抗内生放线菌的筛选及其生防效果初报

采用皿内对峙试验从175株植物内生放线菌株中筛选具有生防效果的拮抗菌株,然后通过盆栽试验和田间试验,对筛选出的生防菌的生防效果进行了研究。结果表明,皿内对峙试验共筛选出对番茄叶霉病菌和番茄早疫病菌、番茄灰霉病菌有拮抗作用的内生放线菌株26株;菌株BAR1-5对番茄叶霉病的防治效果最佳,相对防效达到49.7%,接种番茄叶霉菌前和接种后喷施BAR1-5发酵上清液的相对防效分别达到62.6%和50.6%;BAR1-5菌株发酵液原液和5倍液的相对防效分别达到42.5%和33.1%。试验结果表明,菌株BAR1-5对番茄叶霉病具有较好的防治效果,是1株很有生防潜力的内生放线菌株。

Screening of antagonistic endophytic actinomycetes against tomato Pathogens and biocontrol effect on tomato leaf mould

175 endophytic actinomycetes were screened as the potential biocontrol agent in plate.Then through greenhouse experiment and field experiment,the biocontrol effect of screened strain was studied.The result showed that 26 endophytic actinomycetes had antagonistic action against the pathogen Fulvia fulva,Botrytis cinerea and Alternaria solani in plates.In greenhouse experiment,strain BAR1-5 was the most effective strain against tomato leaf mould,and the control effect of the fermented supernatant after inoculation and before inoculate reached 50.6% and 62.6%,respectively.In field experiment,the control effect of fermentation production treatment and its 5 times dilute treatment reached 42.5% and 33.1%,respectively.The result indicated that strain BAR1-5 was really a potential biocontrol endophytic actinomycete.