ND-EDS-76异源二联油苗田间试验研究

鸭源ＮＤＶ－Ｄ１０毒株与ＥＤＳ－７６ＧＣ２每株混合接种同一鸭胚，培养９６ ｈ收集尿囊液，制成ＮＤ－ＥＤＳ－７６异源二联油乳剂灭活疫苗，然后与普通二联油乳剂灭活疫苗接种同一鸡群，在 １０，２０，３０，１８０，３６０ ｄ分别检测抗体滴度并进行比较，结果差异不显著。在免疫后 ３０，１８０，３６０ ｄ随机取鸡各 ６０只为 １组，用 ＮＤＶ－Ｆ４８Ｅ９和 ＥＤＳ－７６ＧＣ２强毒株进行攻毒保护试验并进行比较，结果表明两种疫苗在 ３０，１８０ ｄ免疫保护率均为 １００％，３６０ ｄ对ＮＤ和ＥＤＳ－７６的免疫保护率分别为９２％和８７％以上．对临床２００万只开产前１２０日龄左右蛋鸡群免疫接种ＮＤ－ＥＤＳ－７６异源二联油乳剂灭活疫苗，可有效地保护整个产蛋期对ＮＤ和ＥＤＳ－７６强毒的攻击。

Field test on oil emulsion inactive duck origin ND-ESD-76 bivalent combinet vaccine

Duck origin NDV-D10 and EDS-76GC2 virus strains were propagated by the same embryos and harvested from embryo allantoic fluid after 96 h culture,and used to prepare oil emulsion inactivated ND-EDS-76 bivalent combined vaccine. Two groups of chicken were vaccinated with the newly developed vaccine and ordinary oil emulsion inactivated bivalend combined vaccine respectively,and the difference of antibody titres between the two groups were not significant. The challenge NDV-F48E9 and EDS-76GC2 virulent strains both acted at 30, 180 and 360 d post-immunization. The two vaccinated groups with 60 birds each,selected randomly,showed 100% protectivity at the first two challenge and over 97% and 87% protectivity at last challenge,but not significant difference. The clinical inoculation of layers aged about 120 d with oil emulsion inactivated duck origin ND-EDS-76 bivalent combined vaccine to 2 million single chicken could obtain excellent immune effects of prevent two corresponding field virulent virus infection throughout the laying period.