| 葡萄无核基因RAPD标记的序列分析 |
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| 引用本文： | 王跃进,Lamikanra O.葡萄无核基因RAPD标记的序列分析[J].西北农林科技大学学报(社会科学版),1997,25(4):1-5. |
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| 作者姓名： | 王跃进 Lamikanra O |
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| 作者单位： | 西北农业大学园艺系，美国佛罗里达农工大学葡萄研究中心 |
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| 摘 要： | 报道了用ＱｉａｇｅｎＫｉｔ纯化ＲＡＰＤ遗传标记，用细菌质粒Ｍ１３克隆ＲＡＰＤ标记的ＤＮＡ片段，采用自动荧光ＤＮＡ测序仪（型号３７３Ａ）测定了该ＤＮＡ片段的核苷酸组成及其排列顺序。无核白ＲＡＰＤ标记由５１９对核苷酸及其特定序列组成。因为无核白是无核基因的原始供体，作者认为所获无核白ＲＡＰＤ标记的ＤＮＡ序列，可以作为合成探针的基础，用于检测无核葡萄育种和无核品种的无核性。
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| 关 键 词： | 葡萄无核基因 RAPD标记 DNA序列 |
Analysis of Sequencing the RAPD Marker Linked to Seedless Genes in Grapes |
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| Wang Yuejin,Lamikanra O.Analysis of Sequencing the RAPD Marker Linked to Seedless Genes in Grapes[J].Journal of Northwest Sci-Tech Univ of Agr and,1997,25(4):1-5. |
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| Authors: | Wang Yuejin Lamikanra O |
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| Institution: | Wang Yuejin 1 Lamikanra O 2 (1 Department of Horticulture,Northwestern Agricultural University, Yangling,Shaanxi 712100,the People’s Republic of China) (2 Center for Viticultural Science,Florida A & M University Talahassee,FL 32307 USA) |
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| Abstract: | This paper presents the sequence of RAPD marker linked to the seedless genes in grapes. The isolation DNA was purified by Qiagen kit and inserted into plasmid PCR TM II(Invitrogene). The sequencing was performed based upon automated flourescent DNA sequencer systems (Model 373A). The marker from the seedless originator Thompson Seedless consists of 519 bp with specific nucleotide sequence. Because of the initial donors of seedless genes from Thompson Seedless, it is suggested that the sequence of Thompson Seedless might be used as basis for synthesizing the probe for detecting seedlessness both in seedless breeding and identification of seedless varieties from it. |
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| Keywords: | Grape seedless genes RAPD marker DNA sequence |
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