睾酮在北方山溪鲵精巢发育周期中的分布

用免疫组织化学方法检测了睾酮(testosterone,T)在北方山溪鲵(Batrachuperustibetanus)精巢发育周期中的变化。结果表明,在北方山溪鲵的精巢中,4月份T定位在次级精原细胞的胞质中;5月份T定位在次级精原细胞的胞核中;6~7月,精母细胞和精子细胞核中呈T阳性反应;8月份,精巢的非成熟区和成熟区散在地出现T的弱阳性位点;9~10月,精巢非成熟区的精原细胞胞质及成熟区精子中有较强的T阳性反应;11月,精原细胞的胞质及精子中的阳性反应减弱。在山溪鲵的生殖周期中,4~11月精巢中始终呈现T阳性反应,而且T的分布呈现出一定的规律性。在4~5月和9~10月,精巢间质细胞和支持细胞中也有T阳性反应。上述结果说明,在山溪鲵精巢中,T对生殖细胞增殖及精子形成起调控作用。

Localization of testosterone in the testis of the stream salamander Batrachuperus tibetanus during the annual reproductive cycle

Location of testosterone (T) in testis of Batrachuperus tibetanus was examined by immunohistochemistry.The results were as follows:testosterone was presented in the cytoplasm of spermatogonia (SPG) in April.It was detected in the nuclear of SPG in May when SPG would become primary spermatocyte(ISPC).During Jun-Jul,T positive reactivity was also detected in the nuclear of spermatocyte (SPC) and spermatid (SPD).In Aug,there were T slight positive reaction dots scattered in the immature region and mature region.In Sep-Oct,T was presented in the cytoplasm of SPG in immature region and spermatozoa (SPZ) in mature region.T positive reactivity weakened in the cytoplasm of SPG and SPZ in Nov.During the annual reproductive cycle,T positive location always presented in the certain part in the testis.T was also detected in Leydig cells (LC) and Sertoli cells (SC) of the testis during Apr-May and Sep-Oct.The result shows that testosterone disperses periodically and regularly in testis of Batrachuperus tibetanus and it plays an regulative role in proliferation of germ cell and spermatogenesis.