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气相色谱仪同时测定药物中的广藿香醇和芳樟醇的方法
引用本文:李军德,胡正禾,赵艳芳,宋吉英.气相色谱仪同时测定药物中的广藿香醇和芳樟醇的方法[J].青岛农业大学学报,2016(4):313-316.
作者姓名:李军德  胡正禾  赵艳芳  宋吉英
作者单位:1. 青岛农业大学化学与药学院,山东 青岛,266109;2. 莱阳卫生学校医院,山东 烟台,265200
基金项目:山东省高等学校青年骨干教师国内访问学者经费资助
摘    要:本文首次用气相色谱法同时检测药物中的广藿香醇和芳樟醇,实验用乙酸乙酯做提取剂,30 ℃~35 ℃的温度范围内提取1 h,采用Agilent DB-5MS石英毛细管柱和氢火焰离子化检测器(FID)进行分析,进样口温度280 ℃;检测器温度280 ℃;柱温使用程序升温(初温60 ℃保持1 min,20 ℃·min-1的速率升至110 ℃保持1 min;2 ℃·min-1的速率升至120 ℃保持0.5 min;再以20 ℃·min-1的速率升至250 ℃保持1 min),不分流进样对广藿香醇和芳樟醇同时进行定性和定量分析,该方法可使广藿香醇和芳樟醇有较好的分离度,可成功用于药物中目标物的分析检测.

关 键 词:气相色谱法  广藿香醇  芳樟醇  分析检测

Detection Method of Patchouli Alcohol and Linalool in Medicine at the Same Time by Gas Chromatography
Abstract:In this paper, a new method was developed for the determination of patchouli alcohol and linalool in medicine at the same time by gas chromatography with splitless injection, and flame ionization detector (FID).The effects of extraction temperature, extraction time were discussed and the conditions were optimized.The samples were agitated in 30~35 ℃ condition for 1 h and ethylacetate was extraction solvent.The gas chromatography instrument fixed a Agilent DB-5MS capillary column.The injector temperature (280 ℃), detector temperature (280 ℃) and The column temperature was programmed.The initial temperature was maintained at 60 ℃ for 1 minute, the temperature was raised to 110 ℃ at the speed of 20 ℃·min-1 and maintained for 1minute;the temperature was raised to 120 ℃ at the speed of 2 ℃·min-1 and maintained for 0.5 minute;and then the temperature was raised to 250 ℃ at the speed of 20 ℃·min-1 and maintained for 1minute.This method was accurate and effective for determination of patchouli alcohol and linalool in medicine.It was suitable for the quality control for patchouli alcohol and linalool.
Keywords:gas chromatography  patchouli alcohol  linalool  detection
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