| 猪捷申病毒中国株3ABC基因的克隆及序列分析 |
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| 引用本文： | 李娟,刘磊,冯力.猪捷申病毒中国株3ABC基因的克隆及序列分析[J].甘肃农业大学学报,2007,42(2):17-21. |
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| 作者姓名： | 李娟 刘磊 冯力 |
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| 作者单位： | 1. 甘肃农业大学动物医学院,甘肃,兰州,730070
2. 中国农业科学院哈尔滨兽医研究所,黑龙江,哈尔滨,150001 |
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| 基金项目： | 中国农业部国家重点生物实验室资助课题(2004) |
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| 摘 要： | 根据已报道的Swine/CH/IMH/03基因序列设计1对引物,从已分离的中国株捷申病毒中提取总RNA,经RT-PCR扩增得到3ABC基因.并将该基因克隆到pMD18-T载体中,经序列测序,并与GenBank上的14种毒株序列进行比对.结果表明:猪捷申病株中国株(PTV-3ABC)与F65毒株、PTV-1毒株、Swine/CH/IMH/03毒株和Talfan毒株的同源性比较高,分别为96.5%,99.5%和99.4%分别为99.4%,氨基酸同源性分别为95.6%,98.8%,98.4%和98.8%.系统发育树表明,此中国株捷申病毒属于PTV-1型.
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| 关 键 词： | 猪捷申病毒 3ABC基因 同源性 序列分析 |
| 文章编号： | 1003-4315（2007）02-0017-05 |
| 修稿时间： | 2006-10-08 |
Cloning and sequencing of 3ABC gene of China strain of porcine teschen virus |
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| LI Juan,LIU Lei,FENG Li.Cloning and sequencing of 3ABC gene of China strain of porcine teschen virus[J].Journal of Gansu Agricultural University,2007,42(2):17-21. |
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| Authors: | LI Juan LIU Lei FENG Li |
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| Institution: | 1. College of Veterinary Medicine,Gansu Agricultural University,Lanzhou 730070,China; 2. Harbin Veterinary Research Institute,CAAS, Harbin 150001,China |
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| Abstract: | Based on the reported sequences of Swine/CH/IMH/03, a pair of primers to amplify the 3ABC gene was designed. The total RNA was extracted from China strain of porcine teschen virus,and the 3ABC gene was amplified by RT- PCR. The products of PCR were cloned into pMD- 18T vector, sequenced and then the sequences were aligned with 14 sequences from GenBank. The results showed that its homology with F65, PTV- 1, Swine/CH/IMH/03 and Talfan strain was very high, showing 96.5 %- 99.4 % identity in nucleotide sequence and 95.6 % -98.8% identity in deduced amion acid sequence. According to the phylogenetic tree,the China strain of teschen virus should belong to PTV- I. |
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| Keywords: | porcine teschen virus 3ABC gene homology sequence analysis |
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