欧拉型藏羊Callipyge基因多态性与生长性状相关性研究

利用PCR-SSCP方法,对215只欧拉型藏羊Callipyge基因序列的多态性进行了检测,并分析了AA和AB基因型与生长性状的相关性.结果表明:Callipyge基因存在AA、BB和AB 3种基因型,其基因型频率分别为0.823 3、0.009 3和0.167 4;测序结果显示:基因多态的产生是在扩增序列的第176bp处发生了C→T的突变;χ2检验表明,该群体在这一位点上处于Hardy-Weinberg平衡状态(P0.05),遗传杂合度和多态信息含量分别为0.168 7和0.154 5.对生长性状(体重、胸围和胸深)的相关性分析表明:AA和AB 2个基因型个体之间生长性状的差异不显著,但AB型有大于AA型的趋势.

Study on correlation between single nucleotide polymorphism of Callipyge gene and growth traits in Oula Tibetan sheep

The genetic polymorphism of Callipyge gene sequence in 215 Oula Tibetan sheep was detected with PCR-SSCP method,and its correlations with growth traits between AA and BB genotypes were analysed.The results indicated that there were three genotypes,and the genotypic frequencies of AA,BB and AB were 0.823 3,0.009 3 and 0.167 4,respectively.The sequencing result showed that the amplified fragment of Callipyge gene was caused by C→T mutation at the 176 bp position.The population was Hardy-Weinberg equilibrium at this locus(P>0.05),and the gene heterozygosity(He)and polymorphism information content(PIC) were 0.168 7 and 0.154 5,respectively.For growth traits,the correlation analysis(body-weight,chest circle and chest depth) showed that the effect of AA and BB genotypes on growth traits was not significant,however the AB genotype had a trend of higher than AA genotype.