| CMC-Na法提取牦牛血红素的工艺研究 |
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| 引用本文： | 刘振斌,张珍,张盛贵,牛黎莉,周芸,胡伟,庞晨.CMC-Na法提取牦牛血红素的工艺研究[J].甘肃农业大学学报,2012,47(4):143-146. |
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| 作者姓名： | 刘振斌 张珍 张盛贵 牛黎莉 周芸 胡伟 庞晨 |
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| 作者单位： | 甘肃农业大学食品科学与工程学院,甘肃兰州,730070 |
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| 摘 要： | 以新鲜牦牛血为原料,采用超声波破碎进行溶血,利用羧甲基纤维素(CMC)的吸附作用提取血红素.结果表明:提取牦牛血红素的最佳的条件为:10mL红血球中,去离子水添加量为40mL,超声波破碎时间为20min,分离pH值为2.0,CMC-Na(3%)的添加量为3mL,该工艺从牦牛血全血中提取的血红素产率为3.028mg/mL.
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| 关 键 词： | CMC-Na法 血红素 牦牛 |
Extraction techniques of heme from yak blood with CMC-Na |
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| LIU Zhen-bin
,
ZHANG Zhen
,
ZHANG Sheng-gui
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NIU Li-li
,
ZHOU Yun
,
HU Wei
,
PANG Chen.Extraction techniques of heme from yak blood with CMC-Na[J].Journal of Gansu Agricultural University,2012,47(4):143-146. |
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| Authors: | LIU Zhen-bin ZHANG Zhen ZHANG Sheng-gui NIU Li-li ZHOU Yun HU Wei PANG Chen |
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| Institution: | (College of Food Science and Engineering,Gansu Agricultural University,Lanzhou 730070,China) |
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| Abstract: | In this paper,hemoglobin was extracted from fresh yak blood by the adsorption of carboxymethyl cellulose(CMC) and sonicating for hemolysis.The results showed that the optimum conditions for extracting was as water 40 mL,sonication for 20 min,pH 2.0,CMC-Na(3%) 3 mL,separating pH 5.5.The yield of hemoglobin could reach 3.028 mg/mL with the extraction techniques. |
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| Keywords: | CMC-Na hemoglobin yak |
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