| 绵羊生长激素基因第4外显子的多态性分析 |
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| 引用本文： | 任春环,吴建平,张利平,张海容.绵羊生长激素基因第4外显子的多态性分析[J].甘肃农业大学学报,2008,43(4). |
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| 作者姓名： | 任春环 吴建平 张利平 张海容 |
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| 作者单位： | 甘肃农业大学动物科学技术学院,甘肃,兰州,730070 |
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| 基金项目： | 甘肃省农业生物技术专项，资助甘肃省农业生物技术专项 |
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| 摘 要： | 采用PCR-SSCP技术检测包括蒙古羊、小尾寒羊、德国美利奴羊以及德美寒杂交一代在内的绵羊生长激素(GH)基因第4外显子的多态性.结果表明:在1188~1502 bp片段上,德国美利奴羊及杂交后代中,AA型个体占多数,而小尾寒羊则以BB型个体为主.对这个片段的纯合型(AA,BB)分别进行克隆测序发现:1188~1502 bp片段上,AA型在第1406位发生了T→C的突变.实验结果证实绵羊生长激素基因第4外显子存在序列多态性.
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| 关 键 词： | 生长激素基因 PCR-SSCP 遗传多态性 绵羊 |
Polymorphism of exon 4 of ovine growth hormone gene |
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| REN Chun-huan,WU Jian-ping,ZHANG Li-ping,ZHANG Hai-rong.Polymorphism of exon 4 of ovine growth hormone gene[J].Journal of Gansu Agricultural University,2008,43(4). |
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| Authors: | REN Chun-huan WU Jian-ping ZHANG Li-ping ZHANG Hai-rong |
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| Abstract: | The polymorphism of ovine growth hormone gene in the 4th exon sequence in sheep including Mongolian,Small-tailed Han sheep,Germanmerino and F1 generation of the Small-tailed Han sheep and Germanmerino were detected by PCR-SSCP with a pair of primers.The results showed that in 1 188～1 502 bp most of the Germanmerino and hybrid generations presented as AA genotype,while most Small-tailed Han sheep presented as BB genotype.The amplified fragments of AA and BB were cloned and sequenced.The result showed that in 1 188-1 502 bp AA genotype had one substitution mutation at 1 406 site(T → C).The results confirmed that there were polymorphisms in the 4th exon region of GH gene. |
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| Keywords: | PCR-SSCP |
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