| 甘农2号杂花苜蓿愈伤组织诱导及体细胞胚和芽分化的研究 |
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| 引用本文： | 胡静,马晖玲,谢俊仁.甘农2号杂花苜蓿愈伤组织诱导及体细胞胚和芽分化的研究[J].甘肃农业大学学报,2007,42(4):87-91. |
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| 作者姓名： | 胡静 马晖玲 谢俊仁 |
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| 作者单位： | 1. 甘肃农业大学草业学院,甘肃,兰州,730070
2. 甘肃农业大学动物医学院,甘肃,兰州,730070 |
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| 基金项目： | 国家科技部奶业专项子课题 |
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| 摘 要： | 以甘农2号杂花苜蓿下胚轴为材料,MS为基本培养基,研究了不同浓度的2,4-D、NAA、KT、BA、蔗糖及2,4-D作用时间对甘农2号杂花苜蓿愈伤组织的诱导、体细胞胚和芽分化的影响.结果表明,3 mg/L 2,4-D+1.5 mg/L NAA为愈伤组织诱导阶段最佳激素组合;2,4-D作用时间以15 d为宜;最佳的芽分化培养基为MS+0.5 mg/L KT+0.1 mg/L NAA+7 g/L琼脂+20 g/L蔗糖.
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| 关 键 词： | 杂花苜蓿 下胚轴 愈伤组织 体细胞胚 芽分化 |
| 文章编号： | 1003-4315（2007）04-0087-05 |
| 修稿时间： | 2006-12-25 |
Callus induction and redifferentiation of Medicago varia Gannong No. 2 |
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| HU Jing,MA Hui-ling,XIE Jun-ren.Callus induction and redifferentiation of Medicago varia Gannong No. 2[J].Journal of Gansu Agricultural University,2007,42(4):87-91. |
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| Authors: | HU Jing MA Hui-ling XIE Jun-ren |
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| Institution: | 1. College of Prataculture,Gansu Agricultural University,Lanzhou 730070,China; 2. College of Veterinary Medicine,Gansu Agricultural University,Lanzhou 730070,China |
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| Abstract: | Taking hypocotyls of Medicago variaGannong No.2 as the explants and MS as basic medium,the effects of different addition concentration of 2,4-D,NAA,KT,BA,sucrose and the inducing time of callus on the callus induction,somatic embryogenesis and shoot differentiation were studied.The result showed that 3 mg/L 2,4-D 1.5 mg/L NAA was the optimum hormone combination,the proper action time of 2,4-D was 15 days,and the optimum culture medium for shoot differentiation was MS 0.5 mg/L KT 0.1 mg/L NAA supplemented with 7 g/L agar and 2 g/L sucrose. |
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| Keywords: | Medicago varia hypocotyl callus somatic embryo shoot differentiation |
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