| 农杆菌介导的紫花苜蓿遗传转化体系的建立与优化 |
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| 引用本文： | 陈晨,曹致中,贺顺姬,乔代蓉,曹毅.农杆菌介导的紫花苜蓿遗传转化体系的建立与优化[J].甘肃农业大学学报,2004,39(5):516-519. |
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| 作者姓名： | 陈晨 曹致中 贺顺姬 乔代蓉 曹毅 |
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| 作者单位： | 1. 甘肃农业大学草业学院,甘肃,兰州,730070
2. 四川大学生命学院,四川,成都,610065 |
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| 基金项目： | 国家高技术发展计划(863 计划)课题:利用基因工程改造苜蓿抗寒耐盐性 |
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| 摘 要： | 以“中苜一号”紫花苜蓿品种作为受体材料,建立了适用于农杆菌介导的转基因组培体系,并对GUS 基因进行转化,经 GUS 组织化学染色,以获得抗性愈伤组织分析为目的,转化优化条件为:叶片预培养 4~5 d,用农杆菌菌液(A600=0.3~0.8)侵染 20 min,然后在培养基上铺一层灭菌滤纸共培养 7 d 后清洗。
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| 关 键 词： | 紫花苜蓿 遗传转化 根癌农杆菌 GUS基因 |
| 文章编号： | 1003-4315(2004)05-0516-04 |
Establishment and optimization of Agrobacterium-mediated transformation of alfalfa (Medicago sativa L.) |
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| CHEN Chen,CAO Zhi-zhong,HE Shun-ji,QIAO Dai-rong,CAO Yi.Establishment and optimization of Agrobacterium-mediated transformation of alfalfa (Medicago sativa L.)[J].Journal of Gansu Agricultural University,2004,39(5):516-519. |
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| Authors: | CHEN Chen CAO Zhi-zhong HE Shun-ji QIAO Dai-rong CAO Yi |
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| Institution: | CHEN Chen1,CAO Zhi-zhong1,HE Shun-ji2,QIAO Dai-rong2,CAO Yi1 |
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| Abstract: | This paper established the regeneration system of somatic embryogenesis for the cultivar alfalfa “Zhongmu NO.1”genetic transformation. Manipulation system was optimized by Km-resistant calli assays and the histological detection of GUS activity, which GUS gene comed from plant expression vector pCAMBIA2301G. The condition of optimization was pre-culture for 4~5 days, infection with Agrobacterium tumefaciens (A600=0.3~0.8) for 20 min and washed after 7 days of culture. |
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| Keywords: | Alfalfa genetic transformation Agrobacterium tumefaciens GUS gene |
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