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| 牦牛GH基因多态性的PCR-SSCP分析 | |
| 引用本文： | 白晶晶,胡江,成述儒,王继卿,罗玉柱.牦牛GH基因多态性的PCR-SSCP分析[J].甘肃农业大学学报,2010,45(1). |
| 作者姓名： | 白晶晶 胡江 成述儒 王继卿 罗玉柱 |
| 作者单位： | 1. 甘肃农业大学动物科学技术学院,甘肃,兰州,730070 2. 甘肃农业大学研究测试中心,甘肃,兰州,730070 3. 甘肃农业大学动物科学技术学院,甘肃,兰州,730070;甘肃农业大学研究测试中心,甘肃,兰州,730070 |
| 基金项目： | 国家科技支撑计划课题(2007BAD52B05);;甘肃省发改委项目(036015) |
| 摘 要： | 采用PCR-SSCP技术对天祝白牦牛和青海高原牦牛的生长激素基因(GH基因)5个外显子及部分内含子序列进行遗传变异分析,以探讨牦牛GH基因的遗传特性.结果表明:2个牦牛群体GH基因第1、第2和第4外显子无多态性,第3内含子1694bp处均发生T→C转换,形成A、B2个等位基因,并检测到AA、AB和BB共3种基因型;天祝白牦牛第5外显子2373bp处发生G→T碱基颠换,形成C、D2个等位基因,检测到CC和CD2种基因型,但碱基改变并未引起氨基酸变化,因此这种碱基转换为沉默突变;天祝白牦牛和青海高原牦牛均为低度多态,经Hardy-Weinberg平衡检验2个牦牛群体均处于非平衡状态. |
| 关 键 词： | 牦牛 GH基因 PCR-SSCP 多态性 |
PCR-SSCP polymorphism of growth hormone gene in the yak |
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| BAI Jing-jing,HUJiang,CHENG Shu-ru,WANG Ji-qing,LUO Yu-zhu.PCR-SSCP polymorphism of growth hormone gene in the yak[J].Journal of Gansu Agricultural University,2010,45(1). | |
| Authors: | BAI Jing-jing HUJiang CHENG Shu-ru WANG Ji-qing LUO Yu-zhu |
| Institution: | 1.College of Animal Science and Technology;Gansu Agricultural University Lanzhou;730070;China;2.Analysis and Research Centre;Gansu Agriculture University;Lanzhou 730070;china |
| Abstract: | Five exons and parts of introns of growth hormone(GH)gene were detected by PCR-SSCP in Tianzhu white yak and Qinghai plateau yak to get molecular genetics information and find the growth character correlation relevance to the molecular.The results indicated that the exon 1,exon 2 and exon 4 did not display polymorphism.The polymorphism of the intron 3 was controlled by A and B alleles and formed three genotypes,namely AA,BB,AB.The result of cloning and sequencing indicated that there was one single nucleoti... |
| Keywords: | yak GH gene PCR-SSCP polymorphism |
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