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甘薯的组织培养
引用本文:雷加容,余金龙,余敖.甘薯的组织培养[J].甘肃农业大学学报,2006,41(1):113-115.
作者姓名:雷加容  余金龙  余敖
作者单位:绵阳市农科所,四川,绵阳,621002
摘    要:以甘薯的叶片为外植体进行组织培养,结果表明:适合甘薯愈伤组织诱导的培养基是MS+KT 2 mg/L+2,4-D 0.5 mg/L和MS+KT 2 mg/L+NAA 0.5 mg/L,适宜愈伤组织分化出根的分化培养基是MS+KT 0.5 mg/L或MS+KT 2 mg/L+NAA 0.5mg/L或MS+KT 2 mg/L+2,4-D 0.5 mg/L,适宜根分化出试管苗的分化培养基为MS+KT 2 mg/L+NAA 0.5 mg/L;增殖培养基为MS;生根的培养基为1/2 MS.

关 键 词:甘薯  组织培养  愈伤组织
文章编号:1003-4315(2006)01-0113-03
收稿时间:2005-04-28
修稿时间:2005-04-28

Tissue culture of Ipomoea batatas
LEI Jia-rong,YU Jin-long,YU Ao.Tissue culture of Ipomoea batatas[J].Journal of Gansu Agricultural University,2006,41(1):113-115.
Authors:LEI Jia-rong  YU Jin-long  YU Ao
Institution:Mianyang Institute of Agricultural Sciences, Mianyang 621002 ,China
Abstract:The leaf blade of Ipomoea batatas were used as explants for tissue culture.The results showed:The optimum medium for callus induction was MS KT 2 mg/L 2,4-D 0.5 mg/L and MS KT 2 mg/L NAA 0.5 mg/L,mediums MS KT 0.5 mg/L,MS KT 2 mg/L NAA 0.5 mg/L or MS KT 2 mg/L 2,4-D 0.5 mg/L were suitable for rooting,MS KT 2 mg/L NAA 0.5 mg/L was the best for seedling production,MS for subculture and 1/2 MS for rooting.
Keywords:Ipomoea batatas  tissue culture  callus
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