| 嗜热脂肪芽孢杆菌中普鲁兰酶的分离纯化 |
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| 引用本文： | 赵淑琴,杨孝朴,胡先望.嗜热脂肪芽孢杆菌中普鲁兰酶的分离纯化[J].甘肃农业大学学报,2011(4):133-138. |
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| 作者姓名： | 赵淑琴 杨孝朴 胡先望 |
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| 作者单位： | 甘肃农业大学动物医学院;甘肃省商业科技研究所; |
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| 基金项目： | 甘肃省技术研究与开发专项计划项目(1004TCYA) |
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| 摘 要： | 嗜热脂肪芽孢杆菌经细胞破碎机破壁后,采用硫酸铵盐析法除杂蛋白,DEAE-32阴离子交换纤维素纯化胞内普鲁兰酶,并通过L9(34)正交试验优化盐析除杂蛋白工艺条件.结果表明:菌体与缓冲液比1:3、硫酸铵饱和度40％、pH值7.0分离效果最佳,其中,pH值对分离效果的影响差异性显著(F＞F0.05)；纯化后的普鲁兰酶比活力...
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| 关 键 词： | 嗜热脂肪芽孢杆菌 普鲁兰酶 分离纯化 |
Purification of pullulanase from Bacillus stearothermophilus |
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| ZHAO Shu-qin,YANG Xiao-pu,HU Xian-wang.Purification of pullulanase from Bacillus stearothermophilus[J].Journal of Gansu Agricultural University,2011(4):133-138. |
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| Authors: | ZHAO Shu-qin YANG Xiao-pu HU Xian-wang |
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| Institution: | ZHAO Shu-qin1,2,YANG Xiao-pu1,HU Xian-wang2 (1.College of Veterinary Medicine,Gansu Agricultural University,Lanzhou 730070,China,2.Business Technology Institute of Gansu Province,Lanzhou 730020,China) |
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| Abstract: | Bacillus stearothermophilus was broken using cell crushing machine,the impurity protein was taken out using ammonium sulfate salting out method.Cell pullulanase was purified using DEAE-32 cellulose anion-exchange.The salting out conditions were optimized through orthogonal test.The results showed that the separate condition was the best when the buffer was three times of volume than bacteria,ammonium sulfate saturation was 40%,pH value was 7.0.pH value had an great effect on the separation,differences were ... |
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| Keywords: | Bacillus stearothermophilus pullulanase purification |
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