首页 | 本学科首页   官方微博 | 高级检索  
     检索      

影响葡萄花药愈伤组织产生和分化频率的因素
引用本文:张利平,曹孜义,李唯.影响葡萄花药愈伤组织产生和分化频率的因素[J].甘肃农业大学学报,1993(3).
作者姓名:张利平  曹孜义  李唯
作者单位:甘肃农业大学农学系,甘肃农业大学农学系,甘肃农业大学农学系 兰州 730070,兰州 730070,兰州 730070
摘    要:葡萄花药培养能产生体细胞胚。体细胞胚的诱导及其频率高低与材料基因型、培养基和培养条件有关。试验结果表明,对花药进行低温和药物处理不能明显地促进体细胞胚胎发生,但通过改进培养基成份,添加AgNO_3和用液-固培养均能促进葡萄花药胚性愈伤组织的产生。供试的14个基因型材料中仅有3个能产生体细胞胚,而体细胞胚仅部分能萌发成正常苗。

关 键 词:葡萄  花药培养  体细胞胚胎发生

Some Factors of Influence on Frequency of Callus Induction and Shoot Differentiation in Grape Anther Culture
Zhang Liping Cao ziyi Li Wei.Some Factors of Influence on Frequency of Callus Induction and Shoot Differentiation in Grape Anther Culture[J].Journal of Gansu Agricultural University,1993(3).
Authors:Zhang Liping Cao ziyi Li Wei
Abstract:Grape somatic embryo could be produced by anther culture. The somatic embryo induction and induction frequency were related to genotypes, media and culture conditions. Sametic embryogenesis had not been enhanced by pretrcat of low temperature and certain chemicals, but the production of embryonic callus could be enhanced by modifying medium component, supplying AgNO_3 and using liquid-solid medium. Only 3 genotypes produced somatic embryos in 14 cultured genotypes. Part of the somatic embryos could regenerate into normal plantlets.
Keywords:grape  anther culture  somatic embryogenesis
本文献已被 CNKI 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号