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山羊痘病毒P32基因的克隆与序列分析
引用本文:马维民,刘湘涛,张强,吴国华,颜新敏,李健,朱海霞,吴润.山羊痘病毒P32基因的克隆与序列分析[J].甘肃农业大学学报,2006,41(4):27-30.
作者姓名:马维民  刘湘涛  张强  吴国华  颜新敏  李健  朱海霞  吴润
作者单位:1. 甘肃农业大学动物医学院,甘肃,兰州,730070;家畜疫病病原生物学国家重点实验室,农业部畜禽病毒学重点开放实验室,甘肃,兰州,730046
2. 家畜疫病病原生物学国家重点实验室,农业部畜禽病毒学重点开放实验室,甘肃,兰州,730046
3. 甘肃农业大学动物医学院,甘肃,兰州,730070
摘    要:应用PCR技术,用特异性引物扩增出羊痘病毒结构蛋白P32基因,连接于pMD18-T载体,转化到JM109感受态细胞,对重组质粒双酶切、PCR鉴定与序列分析得出:P32结构蛋白基因全长969 bp,编码323个氨基酸.与山羊痘病毒和绵羊痘病毒P32基因序列同源性分别达99%和97%;与山羊痘病毒和绵羊痘病毒P32基因编码的氨基酸同源性分别达98.9%和96%.

关 键 词:羊痘病毒  P32基因  克隆  序列分析
文章编号:1003-4315(2006)04-0027-04
收稿时间:2006-02-14
修稿时间:2006-02-14

Cloning and sequence analysis of P32 gene of goat capripoxvirus
MA Wei-min,LIU Xiang-tao,ZHANG Qiang,WU Guo-hua,YAN Xin-min,LI Jian,ZHU Hai-xia,WU Run.Cloning and sequence analysis of P32 gene of goat capripoxvirus[J].Journal of Gansu Agricultural University,2006,41(4):27-30.
Authors:MA Wei-min  LIU Xiang-tao  ZHANG Qiang  WU Guo-hua  YAN Xin-min  LI Jian  ZHU Hai-xia  WU Run
Institution:1. College of Veterinary Medicine,Gansu Agricultural University,Lanzhou 730070; 2. State Key Laboratory of Veterinary Etiological Biology,Key Laboratory of Animal Virology of Ministry of Agriculture, Lanzhou 730046,China
Abstract:The structural protein P32 gene of goat capripoxvirus was amplified by PCR method using specific primers.The P32 gene was ligated with pMD18-T vector and transformed into competent cell JM109.The recombinant plasimid was identified by restriction enzyme and PCR.Sequence analysis indicated that the full-length of the structural protein P32 gene was 969 bp and the deduced amino acid was 323.Homology analysis showed that the sequence of P32 gene shared 99 % and 97 % homology with that of goat capripoxvirus and sheep capripoxvirus which have already published,and the homology of deduced amino acid sequence shared 98.9 % and 96 %,respectively.
Keywords:capripoxvirus  P32 gene  clone  sequence analysis
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