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硝酸银对葡萄愈伤组织生长及其白藜芦醇含量的影响
引用本文:张真,李胜,刘媛,吴兵,李婷,张青松,罗丽媛,薛冲.硝酸银对葡萄愈伤组织生长及其白藜芦醇含量的影响[J].甘肃农业大学学报,2008,43(5).
作者姓名:张真  李胜  刘媛  吴兵  李婷  张青松  罗丽媛  薛冲
作者单位:甘肃农业大学生命科技学院植物组织培养研究室,甘肃,兰州,730070
基金项目:科技部中小企业创业基金,甘肃省科技攻关项目,甘肃省高校研究生导师项目,兰州市高层次人才资助项目
摘    要:以‘黑比诺’葡萄叶柄诱导并继代10个月的愈伤组织为试验材料,通过在培养基中(B5+1.0 mg/L KT)分别添加0、1、5、10、20、30 mg/L不同浓度的AgNO3,研究了AgNO3对愈伤组织生长和白藜芦醇累积的效应,结果表明,除5 mg/L AgNO3外,其他几种浓度处理均对愈伤组织的生长产生抑制作用;添加10 mg/L的AgNO3对愈伤组织中白藜芦醇的累积效果最佳,含量达17.884μg/g,高出对照3.6倍;但AgNO3浓度过高(30 mg/L)时,白藜芦醇含量明显下降;当浓度为5 mg/L时,白藜芦醇的产量最大,为18.878μg/瓶,是对照的4.7倍.

关 键 词:硝酸银  白藜芦醇  毒害  葡萄

Effect of silver nitrate on the multiplication of the callus from grapes cv.'Pinot Noir'and its resveratrol production
ZHANG Zhen,LI Sheng,LIU Yuan,WU Bing,LI Ting,ZHANG Qing-song,LUO Li-yuan,XUE Chong.Effect of silver nitrate on the multiplication of the callus from grapes cv.''Pinot Noir''and its resveratrol production[J].Journal of Gansu Agricultural University,2008,43(5).
Authors:ZHANG Zhen  LI Sheng  LIU Yuan  WU Bing  LI Ting  ZHANG Qing-song  LUO Li-yuan  XUE Chong
Abstract:The subcultured callus induced from grapes cv'Pinot Noir'stem was used as the experimental material in vitro,and the effect of silver nitrate stress with different concentration at 0,1,5,10,20 and 30 mg/L on the callus multiplication and resveratrol production were studied in this paper.The results showed that silver nitrate at different concentrations inhabited the growth of the callus except treatment at 5 mg/L.Adding 10 mg/L silver nitrate promoted the accumulation of resveratrol,the content reached 17.884 μg/g,3.6 times higher than that of the control.Resveratrol production reached the maximum with 18.878 μg/flask when silver nitrate was added at 5 mg/L,4.7 times more than the control.
Keywords:silver nitrate  resveratrol  poison  grapes
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