绵羊卵巢卵母细胞体外成熟培养的研究

研究了绵羊卵巢卵母细胞体外成熟培养,并就影响体外成熟的因素进行了探讨。试验表明:添加10%和15%的胎牛血清(FCS)绵羊卵巢卵母细胞的体外成熟率(81.25%和83.75%)明显高于不添加FCS的对照组的卵母细胞成熟率(65.00%);在基础培养基中10%的FCS存在的条件下,添加一定质量分数的促卵泡素(FSH)和促黄体素(LH)时,卵母细胞的体外成熟率明显高于不添加FSH和LH的对照组的绵羊卵母细胞的体外成熟率;培养24 h和26h卵母细胞体外成熟率(75.00%和80.00%)明显高于培养20 h的卵母细胞体外成熟率(63.30%);从3~6 mm卵泡中抽取的卵母细胞体外成熟率(81.50%)明显高于从1~3 mm卵泡中抽取的卵母细胞的体外成熟率(53.30%)。

Study on the oocytes IVM in sheep

The present study was carried out to research the oocyte IVM in sheep and probed into the factors of influencing the oocytes IVM in sheep.The ovaries were collected from the slaughterhouse in Lanzhou.It was found that the rate(81.25 % and 83.75 %) of the IVM which added 10 % and 15 % FCS to the basic medium was higher than the rate(65.00 %))of the IVM which did not add FCS to the basic medium.Under the condition of 10 % FCS existing in the basic medium,when added FSH and LH to the basic medium,the rate of the IVM was higher than the rate of the IVM the group which free FSH and LH.It was proved that the rate(75.00 % and 80.00 %)of the IVM when the oocytes was cultured 24 and 26 hours was higher than the rate(63.30 %) of the IVM when the oocytes was cultured 20 hours.It was found that the rate(81.50 %) of the IVM of oocytes gotten from 3～ 6 mm follicular were higher than the rate(53.30 %) of the IVM of oocytes gotten from 1～ 3 mm follicular.