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| 小鼠单个冷冻胚胎银染mRNA差异显示方法的建立 | |
| 引用本文： | 李拥军,敖红,孙桂金,赵有璋.小鼠单个冷冻胚胎银染mRNA差异显示方法的建立[J].甘肃农业大学学报,2005,40(4):432-436. |
| 作者姓名： | 李拥军 敖红 孙桂金 赵有璋 |
| 作者单位： | 1. 甘肃农业大学动物科学技术学院,甘肃,兰州730070;中国农业科学院畜牧研究所,北京,100094;扬州大学动物科学与技术学院,浙江,扬州,225009 2. 中国农业科学院畜牧研究所,北京,100094 3. 中国农业科学院畜牧研究所,北京,100094;山东农业大学动物科学与技术学院,山东,泰安,271018 4. 甘肃农业大学动物科学技术学院,甘肃,兰州730070 |
| 基金项目： | 国家自然科学基金资助项目（项目编号：30270162）和北京市自然科学基金资助项目（项目编号：62032019）. |
| 摘 要： | 为建立单个冷冻胚胎银染mRNA差异显示方法,用小鼠单个4C鲜胚和冻胚RT—PCR产物,验证了冻胚和鲜胚实验结果的一致性。并从小鼠4C和8C冻胚的银染聚丙烯酰胺凝胶中回收了8C期的特异条带,通过再扩增、同收、克隆及酶切鉴定,克隆到了小鼠8C期胚胎差异表达的小鼠RP23—20A6基因。结果表明,所研究建立的单个冻胚银染mRNA差异显示方法具有可行性和有效性,可以用于动物早期胚胎基因表达的研究。 |
| 关 键 词： | 小鼠 冷冻胚胎 单胚差显技术 银染法 基因表达 |
| 文章编号： | 1003-4315（2005）04-0432-05 |
| 收稿时间： | 2005-01-06 |
| 修稿时间： | 2005-01-06 |
Establishment of silver staining mRNA differential display using single mouse freeze embryo |
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| LI Yong-jun,AO Hong,SUN Gui-jin,ZHAO You-zhang.Establishment of silver staining mRNA differential display using single mouse freeze embryo[J].Journal of Gansu Agricultural University,2005,40(4):432-436. | |
| Authors: | LI Yong-jun AO Hong SUN Gui-jin ZHAO You-zhang |
| Abstract: | The silver staining mRNA differential display was established using RT-PCR product from single mouse 4-cell fresh embryo and freeze embryo respectively, the results were same. One special band in 8-cell stage was recovered from the silver staining polyacrylamide gel of mouse 4-cell and 8-cell freeze embryos, and the obtained special band was reamplified, recovered, cloned and enzyme digested, then mouse RP23-20A6 gene expressed by this band was gained. The results of this study indicated that the method had been established was credible and practical, and it could be used for the study of gene expression about early embryo. |
| Keywords: | mouse freeze embryo single embryo mRNA differential display silver staining gene expression |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |