培养基和培养条件对葡萄试管苗生根的影响

以矢芙罗莎等4种葡萄试管苗为材料,研究了蔗糖浓度、琼脂浓度、pH、光强及温度对葡萄试管苗生根的影响.结果表明:蔗糖浓度为15~20 g/L对葡萄试管苗的生根最有利;pH值对试管苗平均根系数量、根粗、根长和根冠比影响显著,生根数与平均根长随pH值升高而减小,平均根粗与根冠比随pH值的增大而增大;琼脂浓度显著影响葡萄试管苗生根,随着琼脂浓度的增加,根源基产生时间延迟,平均根长和根冠比降低,而平均根粗和生根数增加;生根数在3种温度条件下无显著差异,平均根粗随温度的升高略有下降,平均根长和根冠比随培养温度的升高而增加,在35℃时降低;平均生根数、根长、根冠比和根粗均随光照强度的增强而增大.

Effects of different media and culture conditions on rooting in vitro of test-tube plantlets of Vitis vinifera L.

Effects of different culture conditions including different concentration of sucrose and agar added in media,different pH values, culture temperature and light intension on rooting in vitro of test-tube plantlets of Vitis vinifera L.were carried out.The results showed that the optimum concentration of sucrose for rooting was 15～20 g/L.pH value had significant effects on root number,root diameter,root length and root-shoot ratio of test-tube plantlets cultured with different media,pH value increased,root number and average root length decreased,root diameter and root-shoot ratio increased.Concentration of agar significantly affected the rooting of test-tube plantlets,the high agar concentration was the later root primordium produced,meanwhile,as concentration of agar increased,average root length and root-shoot ratio decreased,average root diameter and root number increased.Root number showed no significant differences among three temperature treatments,while the temperature increased,average root diameter decreased a little,average root length and root-shoot ratio increased first,but when temperature reached to 35 ℃,they decreased.With the increasing of light intension,number,length and diameter of root and root-shoot ratio were all increased.