| 苍耳素Ⅲ-1的分离纯化及鉴定 |
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| 引用本文： | 张帆,杨顺义,张新虎,沈慧敏.苍耳素Ⅲ-1的分离纯化及鉴定[J].甘肃农业大学学报,2008,43(4). |
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| 作者姓名： | 张帆 杨顺义 张新虎 沈慧敏 |
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| 作者单位： | 甘肃农业大学草业学院,甘肃,兰州,730070 |
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| 基金项目： | 甘肃省农业生物技术研究与应用开发项目，甘肃省教育厅科研项目 |
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| 摘 要： | 以黄瓜枯萎病菌为供试菌,采用生物活性示踪法,对苍耳丙酮提取物Ⅲ-1的抑菌成分进行分离.结果表明:苍耳丙酮提取液Ⅲ-1对黄瓜枯萎病菌有抑制作用的有效成分主要集中在2~7、9~11和19、21等10个流份中,其中流份5的抑制率达到51.00%;通过薄层层析分离和GC-MS联用仪鉴定,发现苍耳素Ⅲ-1的主要抑菌成分有呋喃甲醛、5-羟甲基糠醛、4-羟基-4-甲基-2-戊酮等.
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| 关 键 词： | 苍耳素Ⅲ-1 抑菌成分 分离纯化 鉴定 |
Isolation and identification of extract Ⅲ-1 from Xanthium sibiricum |
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| ZHANG Fan,YANG Shun-yi,ZHANG Xin-hu,SHEN Hui-min.Isolation and identification of extract Ⅲ-1 from Xanthium sibiricum[J].Journal of Gansu Agricultural University,2008,43(4). |
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| Authors: | ZHANG Fan YANG Shun-yi ZHANG Xin-hu SHEN Hui-min |
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| Abstract: | The antifungal activity of the extract Ⅲ-1 from Xanthium sibiricumwas was tested with Fusarium oxysporium.The antifungal composition of the acetone extraction Ⅲ-1 was isolated primarily with column chromatography.The results showed that the antifungal activity appeared in fractions of 2 to 7,9 to 11,and 19,and 21.The fifth fraction had the highest growth inhibition rate with 51.00 %.By thin layer chromatography(TLC) isolation and gas chromatography and mass spectroscopy(GC-MS) identification antifungal,the primary antifungal ingredients were 2-furancarboxalde,5-(hydroxymethyl)-2-furaldehyde,diacetone alcohol. |
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| Keywords: | extract Ⅲ-1 from Xanthium sibiricum antifungal ingredients isolation identification |
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